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Members of Archaea & Bacteria that like to live in extreme environments are known as ____.
Extremophiles
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Woese originally lumped archaea & bacteria together in the same category & called them Archaebacteria. What are the 2 reasons that has since been changed?
- Not all members are extremophiles or related to them
- They are not Bacteria--they are archaea
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Which phyla is the largest & most diverse grp of species, includes many familiar gram-neg genera such as Escherichia as well as recognized human pathogens such as Shigella, Salmonella, Neisseria, Yersinia & Vibrio?
Proteobacteria
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Animals having jointed appendages & a segmented body are known as ____.
Arthropods
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Which phyla contains the following: Gram Pos, Thick "skin" cell wall structure Include Bacillus, Clostridium, Staph, Strep, Mycoplasma
Firmicutes
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What are 2 characteristics of actinobacteria & 2 important genus?
- Gram-pos
- Branched Filaments resembling fungi
- Streptomyces → antibiotics
- Mycobacterium → TB
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What species is phylogenetically related to the gram-pos species, Can exist as unicellular, filamentous or colonial forms Was once known as blue green algae but may be black, yellow, green, red
Cyanobacteria
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A sudden increase in the numbers of cells of an organism in an environment in known as ____.
Blooms
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What phylum of bacteria is responsible for the periodic redness of the Red Sea & the "oxygen revolution?"
Blooms of cyanobacteria
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What phylum are characterized by causing chlamydia, are so small that they cannot be seen with light microscope & are cultivated only within living cells?
Chlamydiae
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What phylum contains more than 340 gram-neg species w/unique cell body that coils into long helix & moves in a corkscrew pattern?
Spirochaetes
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What are 2 common spirochaetes?
- Treponema pallidum responsible for syphilis
- Borrelia responsible for Lyme disease & relapsing fever
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Bacteria that grow in high temperature areas are known as ____.
Hyperthermophiles
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The grp euryarchaeota contains methane producing organisms that are killed by oxygen are called ____.
Methanogens
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Euryarchaeota that require O₂ for energy metabolism & need high concen of salt to grow & reproduce are called ____ ____.
Extreme Halophiles
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Histone proteins are not present in Bacteria but are in ___ & ___.
Archaea & Eukarya
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Peptidoglycan cell walls are present in ___ but not in archaea or ___.
-
Bacteria & Eukarya cannot survive in environments exceeding 100⁰C however ___ can.
Archaea
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A bacterial cell with a rod shape is called a ___.
Bacillus
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Most bacillus occur singly, in prs called ___ & or in a long chain called ____.
- Diplobacillus
- Streptobacillus
-
A spherically shaped bacterial cell is known as a ___.
Coccus
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Bacillus range in length from ___μm - ___μm
0.5 - 20
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Coccus tend to be ___ μm - ___μm in diameter.
0.5 - 1.0
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Coccus can be single, ____ (pair), ___ (grp of 4), ____ (cluster) or ___ (chain).
- Diplococcus
- Tetrad
- Staphylococcus
- Streptococcus
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What are the 3 forms of spiral bacteria?
- Vibrio
- Spirillum
- Spirochete
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The ___ is a curved rod that resembles a comma.
Vibrio
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The spiral form ___ has a helical shape w/a thick rigid cell wall & flagella that assist in mvmt.
Spirillum
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The spiral shaped form known as ____ has a thin, flexible cell walnut no flagella in the traditional sense.
Spirochete
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How do spirochete move?
By contractions of endoflagella that run the length of the cell
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A pathogen produced molecule or structure that allows the cell to invade or evade the immune sys & possibly cause disease is known as a ___ ___.
Virulence Factor
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Numerous short thin fibers composed of protein that protrude fr most gram-neg bacteria are called ____.
Pili
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The purpose of pili is to what?
Attach cells to surfaces forming biofilms w/special adhesive molecules called adhesins
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What form of pili est contact between appropriate cells facilitating transfer of genetic material fr donor to recipient thru conjugation?
Conjugation Pili
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What makes conjugation pili different fr adhesion pill?
- They're longer
- Only 1 or a few exist per cell
-
Many bacterial & archaeal cells are motile by using remarkable "nannomachines" called ____.
Flagella
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What are the 3 components of a flagellum?
- Helical filament
- Hook
- Basal Body
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Flagella propel bacteria toward nutrient sources or attractants in a process known as ____.
Chemotaxis
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Some bacterial cells can move about w/o flagella, moving along the long axis of bacillus or filamentous shaped cells. This mvmt is called?
Gliding motility
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One that compares the chem environment & concentration fr 1 moment to the next utilizes a ____ ____ system.
Temporel Sensing
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Many bacterial cells secrete an adhering layer of polysaccharides or polysaccharides & sm proteins called the ____.
Glycocalyx
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If the glycocalyx is thick & covalently bound to the cell it is known as a ____.
Capsule
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A thinner, loosely attached layer of glycocalyx is referred to as a ___ ___.
Slime Layer
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What is the role of the glycocalyx?
- Buffer between cell & external environment
- Allows cells to attach to surfaces
- Protection fr desiccation
-
The ___ ___ provides structure & protects against cel lysis.
Cell Wall
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A network of disaccharide chains (glycan Strands) cross linked by short peptides is called a ____.
Peptidoglycan
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Each disaccharide of a peptidoglycan contains what?
- N-acetylglucosamine (NAG)
- N-acetylmuramic acid (NAM)
-
A poison that can activate inflammatory responses, leading to high fever, shock & organ failure is called an ____.
Endotoxin
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___-___ bacterial cells have a very thick, rigid peptidoglycan cell wall.
Gram-Positive
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Gram-positive cell walls also contain a sugar-alcohol called ___ ___ that is essential for cell viability.
Teichoic Acid
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Enzymes that break bonds in the peptidoglycan, thereby causing lysis of the cell are called ___ ___.
Autolytic enzymes
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What 2 things make gram-negative cell walls different from gram-positive?
- Peptidoglycan layer is only 1 or 2 layers of glycan
- There is no teichoic acid present
-
What is present in a gram-neg cell wall that makes it unique?
Presence of an outer membrane separated fr the cell membrane by a gap called a periplasm
-
What is contained in the periplasm?
- Digestive enzymes
- Transport proteins
-
In a gram-neg cell the inner half of the outer membrane is composed phospholipids while the outer half is composed of what?
Lipopolysaccharides (LPS)
-
A ____ consists of a polysaccharide attached to a unique lipid molecule known as lipid A.
Lipopolysaccharide
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What are porins?
Proteins that form pores in the outer membrane of a gram-neg cell through which sm hydrophilic molecules pass into the periplasm
-
What is the most common cell wall among the archaea?
A surface layer called the S-Layer
-
What does the S-Layer consist of and where is it found?
- Hexagonal patterns of protein or glycoprotein that self-assemble into a crystalline lattice pattern 5nm to 25nm thick.
- S-Layer is most common cell wall in archaea
-
A ___ or ___ membrane is a universal structure that separates external fr internal environments.
Cell or Plasma
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Cells walls in archaea differ fr those in bacteria & eukarya how?
Hydrophobic lipid tails are attached to glycerol heads via ether linkages rather than esther linkages
-
The bacterial genus mycobacterium is related to gram pos bacteria however their cell wall is composed of a waxy lipid that is arranged in 2 layers covalently attached to the underlying peptidoglycan. This waxy lipid is known as?
Mycolic acid
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Gram-pos or Gram-neg cell walls contain peptidoglycan?
- Gram-pos - yes - thick
- Gram-neg - yes - thin
-
Which gram walls contain teichoic acids?
Gram-pos
-
Which gram cell walls utilize and outer membrane?
Gram-neg
-
Which gram cell walls contain lippopolusaccharides (LPS)?
Gram-neg
-
Which gram cell walls have porin proteins?
Gram-neg
-
Which gram cell walls utilize a periplasm?
Gram-neg
-
Explain the lipid monolayer found in archaeal cells.
Fatty acid tails absent fr membranes & replaced by repeating 5-C units linked end to end to form lipid tails longer than fatty acid tails resulting in a monolayer rather than a bilayer
-
What is the advantage to the monolayer found in archaeal cell walls?
It prevents the layer fr peeling in 2 in hyperthermophiles
-
Genetic elements capable of incorporating & transferring genetic info are called ____.
Vectors
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Pili are composed of protein & found primarily & for what purpose?
Gram-neg bacteria & used for attachment & genetic transfer
-
Flagella are composed of ___ & used for ___ & are present in many rods & spirilla; few cocci; vary in # & placement.
-
A structure composed of polysaccharides & sm proteins that provides a buffer to environment & helps w/attachment is called the ___.
Glycocalyx
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The glycocalyx creates a capsule or slime layer does what?
- Contributes to disease development
- Found in plaque bacteria & biofilms
-
What are the 3 functions of a cell wall?
- Cell protection
- Shape determination
- Cell lysis prevention
-
What internal structure exists as a single, closed loop chromosome, contains DNA & is site of essential genes?
Nucleoid
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Plasmids are composed of ___ & are the site of nonessential genes & ____.
-
Which one of the following is NOT a genus within the gram-pos bacteria -- Staphlococcus, Mathanogens, Mycoplasma, Bacillus & Clostridium?
Mycoplasma
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The domain archaea includes all the following grps EXCEPT the (mycoplasms, Extreme Halophiles, Crenarchaeota, Euryarchaeota)
Mycoplasms
-
Spherical bacteria in chains would be referred to as a ____ arrangement.
Streptococcus
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Intracellular organization in bacterial & archaeal species is centered around what 3 processes?
- Sensing & responding to the environment
- Compartmentation of metabolism
- Growth & reproduction
-
Which 1 of the following does NOT apply to pili (made of protein, allow for attachment, facilitate nutrient transport, contain adhesions)?
Facilitate nutrient transport
-
Capsules are similar to pili b/c both (contain DNA, Are made of protein, Contain dextran fibers, Permit attachment to surfaces)
Permit attachment to surfaces
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Gram neg bacteria would stain ___ w/the gram stain & have ____ in the cell wall.
- Orange-Red
- Lipopolysaccharides
-
The cell membrane of archaeal cells contains (A monolayer, Sterols, Ester linkages, All are correct).
All are correct
-
The mvmt of glucose into a cell occurs via . . .
Facilitated Diffusion
-
When comparing bacterial & archaeal cell membranes only bacterial cell membranes have (Three layers of phospholipids, phospholipid bilayer, are fluid, have ether linkages)?
Phospholipid Bilayer
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Which of these is NOT true about the nucleoid (It contains a DNA chromosome, Represents a nonmembranous subcompartment, Represents an area devoid of ribosomes, Contains nonessential genetic info)?
Contains nonessential genetic info
-
Plasmids may carry ___ ___ genes.
Antibiotic resistance
-
Which of the following is NOT a subcompartment found in bacterial cells (Microcompartments, Volutin, Ribosomes, Mitochondria)?
Mitochondria
-
A polyprotein shell 100-200nm in diameter surrounding various types of enzymes is known as a ____.
Microcompartment
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Microcompartments in cyanobacteria are called ___ & serve what function?
- Carboxysomes
- Enhance CO₂ fixation
-
Cytoplasmic structures in the cytoplasm that store nutrients or the monomers of cellular structures are known as ____.
Inclusions
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An inclusion found in diphtheria bacilli contain metachromatic granules or ___, which are deposits of polyphosphate along w/calcium & other ions.
Voluntin
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An inclusion that is a cytoplasmic compartment built fr a water tight protein shell that generates & regulates buoyancy is what?
Gas Vesicle
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The bacterial cytoskeleton (A. transports vesicles B. helps determine cell shape C. is organized identical to its eukaryotic counterpart D. centers the nucleoid)
Helps to determine cell shape
-
The bacterial cell is capable of (A. spatial separation of metabolic processes B. carrying out complex metabolic processes C. subcompartmentalizing biochemical processes D. All)
All
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