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Inoculation of Culture Media
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What are the ways to transfer bacteria?
broth culture to broth media
broth culture to solid media
solid media to solid media
Is media sterile?
yes
What do we use in the lab to create fire?
Bunsen burner
What do we need fire for?
flaming transfer instruments
flaming the lips of the tubes
What are the steps to lighting the Bunsen burner?
turn handle 1/2 turn
ignite with spark igniter
after lighting, adjust needle valve and air vent to achieve the ideal flame
How do we hold the inoculating loop?
like a pencil
Why do we sterilize the inoculating loop starting from the top and moving towards the tip?
limits aerosol production by allowing the loop to heat up more slowly than if it were thrust into the flame immediately
Do we need to let the loop cool before touching the culture?
yes, we don't want to burn and kill the bacteria
Author
kris10leejmu
ID
193066
Card Set
Inoculation of Culture Media
Description
Microbiology
Updated
2013-01-19T22:48:24Z
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