Setting Up a Micro Lab

  1. Describe an ideal micro lab.
    • separate room
    • good lighting
    • good ventilation
    • washable floors
    • limited traffic
    • 2 tables
    • smooth surfaces
    • gas and electric outlets
    • storage space
    • access to incubator, autoclave, and refrigerator
  2. What are the materials needed for a microbiology culture?
    • media
    • gas outlet
    • Bunsen burner
    • portable propane torch
    • gram stain kit
    • 3% hydrogen peroxide
    • inoculating loops
    • slides
    • sensitivity disks
  3. Which media is used for initial growth and cultivation for further testing?
    Columbia Blood Agar
  4. Which media is used to test for antibiotic sensitivity?
    Mueller-Hinton Agar
  5. What is the gas outlet and Bunsen burner used for?
    sterilizing loops and heat fixing slides
  6. What is rabbit plasma used for?
    coagulase testing for Staphylococcus aureus
  7. What do we use the autoclave for?
    to kill anything that has been cultured in the clinic, including fungal cultures
  8. Can we run materials used for cultures with surgery packs?
    no, run them separately
  9. Why do some clinics use old autoclaves for microbiology?
    because the bags can leak and it can be very messy
  10. What are the advantages to having an in-house microbiology lab?
    • ability to do gram stains
    • faster turn around time and more control over the testing process
    • less chance of contamination from multiple persons handling samples
    • fewer no-growth samples when bacteria are cultured quickly
    • less chance of cultures getting lost
  11. What are the disadvantages of having an in-house micro lab?
    • takes up tech time
    • must have someone who is trained and interested
    • unless you are doing micro regularly, supplies can become outdated and need to be replaced
    • hospitals are typically laden with bacteria that can contaminate samples
Card Set
Setting Up a Micro Lab