MicB 3301-Quiz Material

Study of Ab/Ag interactions

specific Ag binding with Ab

Immunoprecipitation detects soluble Ag with a visible precipitate

• Precipitins are Ab



Uses immunodiffusion to examine the ability of test solutions of Ab and Ag to form stable immune complexes. A ppt line forms on agar between sera and anti-sera that match.

Virulence factors that destruct RBCs and leave alpha or beta hemolysis on BAP

Virulence factors that clot blood plasma and form a protective shield to protect the bacteria from macrophages

Virulence factors that lyse plasma or fibrin clots; frees organism to spread

• Enterotoxins: stimulate GI cells abnormally
• Cytotoxins: kill host via enzymatic attack
• Neurotoxins: interfere with normal nerve fx

determines the production of diptheria toxin via a known specific Corynebacterium diptheriae antitoxin

method for determining lecithinase production: egg-yolk agar is rich in lecithin. Lecithinase C toxin is produced by Clostridium perfringens. It enables the organism to rapidly spread via edema, necrosis, gas production, and gangrene.