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DNA structure
twisted ladder aka double helix, sugar/phosphate frame, nitrogenous bases/base pairs as rungs
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Four bases
- Adenine
- Thymine
- Guanine
- Cytosine
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Nitrogen bases are held together by
Nitrogen bonds
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Steps of replication
- 1. Helicase unzips double helix
- 2. Free floating nucleotides fill in with complimentary bases on the exposed DNA strands
- 3. DNA polymerase fuses free floating nucleotides to complimentary DNA strands
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Nucledotides
Nitrogen base, sugar and phospate floating in nucleus
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Polymerase
A second enzyme needed in replication
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DNA contains
A stored code called the genetic code
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Proteins are the
Key molecules to all the functions in all living organism
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Messanger RNA (mRNA)
Because the DNA can't leave the nucleus it needs an intermediate molecule to take the code from the DNA to the ribosomes
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Info about DNA
- ATGC
- Double stranded
- Deoxyribose sugar
- Very stable
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Info about RNA
- AUGC
- Single stranded
- Ribose sugars
- Unstable
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Transcription
Process of copying DNA into mRNA
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Transcription process
- 1. Cells get signal to make protein
- 2. DNA where gene is located is unwound/split by DNA helicase
- 3. One side of the DNA will be transcribed into mRNA
- 4. Polymerase builds mRNA onto sense strand with complemenatry nucleotides
- 5. mRNA is complete and mRNA breaks away from the DNA/leaves nucleus, DNA goes back together
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Sense Strand
The side of DNA transcribed into mRNA
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Anit-sense strand
Side not used to make mRNA
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Translation
- 1. mRNA attaches the ribosome at its start codon (AUG)
- 2. 1st codons exposed
- 3. Amino acids are floating in the cytoplasm, correct AA needs to get to the ribosome, tRNA brings AA
- 4. Comp. tRNA (with anti-codon for mRNA) brings correct amino acid
- 5. 2nd tRNA brings next amino acid
- 6. First tRNA leaves
- 7. Ribosomes moves over to expose the next codon
- 8. Repeats until reachs stop codon
- 9. Chain released, ribosome/mRNA fall apart
- 10. Protein sent through ER to the golgi for packaging
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Number of amino acids
20 represented by 3 letter abbreciations
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Strains of Cholera
- Classic-rare/treated with cholorine
- El Tor-Most common/slight resistance to cholorine
- Bengal-Deadly/Resistant to cholorine
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What is Kock's Postulates
Criteria by which an organism could be determined to be an infectious agent (Pathogen)
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Koch's postulates
- 1. Microorganism present with disease, absent when organism healthy
- 2. Be able to isolate microorganism
- 3. Cause disease again when placed in different organism
- 4. Reisolate from 2nd organism
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3 types of pathogens
Bacteria, virus, parasites
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Bacteria
Single celled prokaryotic organism. Lack nuclei and mitochondria. Has a cell wall. Found anywhere.
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Bacteria is good at
- Helping animals make vitamins
- Keep soil fertile
- Decompose dead matter
- Help plants get nitrogen
- Carry out processes that gives us cheese/yogurt
- Help us digest
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Bacterial shapes
- Spherical
- Rod shaped
- Helical or spiral
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How to get rid of bacteria
Antibiotics-creating holes in cel wall
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Virusues
Genetic material surrounded by proteins- not cells! They take over cells
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How to get rid of viruses
No miracle drugs, vaccines and treatments
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Parasites
Organisms that live at the expense of a host by using their nutrients
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How parasites harm their hosts
- 1. Mechanical damage
- 2. Posining with toxins
- 3. Robbing host of nutrients
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How to treat a parasite
Drugs primarily interfere with metabolsim of the parasite.
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Codon
unit in messenger RNA consisting of a set of three consecutive nucleotides specifies an amino acid.
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Anti-codon
unit of genetic code: a set of three nucleotides in transfer RNA involved in making protein
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Peptide bond
the chemical bond between carbon and nitrogen in a peptide linkage
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amino acid
make up proteins and are important components of cells.
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epidemic
fast-spreading disease
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pandemic
having widespread effect
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Tpyes of proteins
Enzymes, insuline,
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