DNA molecules formed when segments of DNA from two different sources are combined in vitro
biotechnology
the manipulation of organisms or their components to make useful products
genetic engineering
the direct manipulation of genes for practical purposes
plasmids
small circular DNAmolecules that replicate separately from the bacterial chromosome
use for cloning and insert DNA from another source = recombinant bacterium
bacterial artificial chromosome (BAC) - large plasmids
restriction enzymes
protect the bacterial cell by cutting up foreign DNA from other organisms or phages
restriction fragments
have at least one single stranded end, called a sticky end
c
an be made permanent by the enzyme DNA ligase
cloning vector
original plasmid, defined as a DNA molecule that can carry foreign DNA into a host cell and replicate there
2: multiply rapidly, readily obtained from commercial suppliers
expression vector
a cloning vector that contains a highly active bacterial promoterjust upstream of a restriction site where the eukaryoticgene can be inserted in the correct reading frame