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4 types of Macromolecules
- 1. Carbohydrates
- 2. Lipids
- 3. Protiens
- 4. Nucleic Acids
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Monomer of Carbohydrates
Monosaccharides or Simple Sugars
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Example of a Carbohydrate
Sugars (glucose), Starch, Glycogen, etc..
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Monomer of a Lipid
No real monomer, but made up of C, H, and O atoms
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Example of a Lipid
Unsaturated or Saturated Fats, Phosopholipids, Steroids, Cholesterol, etc..
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Monomer of a Protein
Amino Acids and Polypeptides
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4 Levels of Structure of a Protein
Primary, Secondary, Tertiary, and Quaternary.
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Monomer of a Nucleic Acid
Nucleotides
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Example of a Nucleic Acid
DNA and RNA
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Structure of Phospholipids
A phosphate group, glycerol, and 2 fatty acids
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Function of Phospholipids
Make up the cell membrane and forms bilayer
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Characterisitcs of Prokaryotic Cell
- NO membrane bound organelles
- DNA is in nucleoid region
- Possess a cell wall OUTSIDE of plasma membrane
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General characteristics of Eukaryotic Cell
- Membrane bound organelles
- DNA in nucleus
- do not ALWAYS possess a cell wall
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Characteristics of Eukaryotic ANIMAL Cell
- Lysosome (for enzymes)
- Centrosomes (organizational)
- Flagella (cell mobility)
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Characteristic of Eukaryotic PLANT Cell
- Chloroplasts (photosynthesis)
- Central Vacuole (storage)
- Cell Wall (structure)
- Plasmodesmata (bridge between cells)
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Light Microscopy (LM)
Light passes through the cell sample
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Electron Microscopy (EM)
2 types
- Electrons focused on cell sample
- SEM & TEM
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SEM
- Scanning Electron Microscopy
- Focus on cell surface
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TEM
- Transmission Electron Microscopy
- Focuses inside the cell
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Cell Fractionation
Breaking cell open to purify organelles
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Nuclear Envelope
double membrane structure surrounding the nucleus
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Nuclear Pore Complexes
Pores in envelope with proteins in it
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Chromosomes
A long piece of DNA with a lot of proteins
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Ribosomes
Complexes of rRNA and protein to build protein
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2 Types of Ribosomes
- 1. Free Ribosomes
- 2. Bound Ribosomes
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Endomembrane System
A group of organelles related by physical contact
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How do molecules move around in a cell?
Small vesicles or sacs of membranes
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Endoplasmic Reticulum (ER)
Surrounds the nucleus and has two parts: smooth ER and rough ER
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Describe function of the smooth ER
There are no ribosomes. Synthesizes lipids, detoxifies drugs, and stores calcium.
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Describe the function of the rough ER
It is bounded by protein. It makes protein, acts as a gatekeeper of vesicular transport, makes membrane.
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Structure of the Golgi
A group of flattened stacks (called cisternae). Two sides of the golgi: recieving side from the ER called cis and exporting side called trans.
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Function of the Golgi
- 1. Modifies protein
- 2. Storage
- 3. Organization
- 4. Makes carbohydrates
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Structure of Lysosomes
Sacs of membrane with enzymes in it.
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Function of Lysosomes
- To eat a cell (phagocytosis)
- Digestion of old organelles (autophagy)
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Endosymbiotic Theory
Mitochondria and Chloroplasts came from the engulfment of prokaryotes because they have similar structure.
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Structure of Mitochondrion
A double membrane. Inner membrane is a lipid bilayer that extensively folds. Purpose of folds gives more surface area for enzymes.
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Mitchondra Matrix
Inside inner membrane where free ribosomes are.
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Intermediate Space in Mitochondria
Between inner membrane and outer membrane.
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Function of Mitochondria
Cellular Respiration occurs and production of ATP.
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Structure of Chloroplasts
Double membrane structure that holds thykaloids, granum, and stroma.
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Function of Chloroplasts
Gives green pigment and where photosynthesis occurs.
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Structure of a Peroxisome
Single membrane structure.
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Function of a Peroxisome
- Detoxify harmful compounds
- Break down fatty acids
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Function of the Cytoskeleton
- To support organelles and shape of cell.
- Cell motility
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3 Types of Cytoskeleton Fibers
- 1. Microtubules
- 2. Microfilaments
- 3. Intermediate Filaments
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Structure of Microtubules
- Biggest
- Tubulin dimers that form a hollow tube.
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Function of Microtubules
Cell motility, support cell shape and organelles movements (acts as tracks they move along).
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Structure of Microfilaments
- Smallest
- Actins that make up 2 intertwined strands.
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Function of Microfilaments
Maintain or changes the shape of cell.
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Structure of Intermediate Filaments
- Size inbetween Microtubules and Microfilaments
- Fibers of protein coiled into thicker cables.
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Function of Intermediate Filaments
Maintains cell shape because very stable.
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Structure of Cell Membrane
Phospholipids that make a bilayer. Also contains proteins, carbohydrates, or cholesterol.
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Function of Cell Membrane
Control and regulate the passage of materials (permeability), foundation for cytoskeleton, and recieve or transmit information.
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Fluid Mosaic Model
Phospholipids that make up a bilayer with proteins randomly embedded in it.
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Definition of Membrane Fluidity
Membrane bilayer could move around = Dynamic
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Phosopholipids can move two ways:
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Unsaturated Hydrocarbons
Contains double bonds between carbons. Causes "kinks" and makes less compact = more fluidity.
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Saturated Hydrocarbons
Has no double bonds which makes more compact molecules = less fluidity.
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Definition of Membrane Permeability
Regulation/Trafficing of molecules entering or exiting the cell.
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Molecules easy to pass through membrane..
Hydrophobic, small, nonpolar molecules.
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Molecules difficult to pass through membrane..
Hydrophilic, long, polar molecules.
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Diffusion
Random movement of molecules trying to reach equilibrium. Molecules will move from high to low concentration ("down the gradient").
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Passive Transport
Diffusion across membrane without the use of energy. Means that molecules can move easily across the membrane.
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Osmosis
- Diffusion of water molecules across a selectively permeable membrane. Water molecules will move from low to high concentration of solute.

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Definition of Tonicity
Ability of a solution to cause a cell to lose or gain water.
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Hypotonic Solution
- There is a low concentration of water molecules in the cell.

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Isotonic Solution
- There is the same concentration of water molecules in the cell as outside the cell.

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Hypertonic Solution
- There is a high concentration of water molecules in the cell.

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What happens to a Hypotonic Cell?
- Water will move into the cell and could cause it to burst.

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What happens to Isotonic Cells?
- Nothing, there is equal amount of water inside and outside of the cell. Water will equally move in and out.

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What happens to Hypertonic Cells?
Water could move out of the cell and it could cause it to shrival up.
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Facilitated Diffusion
- Diffusion that requires proteins to move solute down its concentration gradien without the input of energy.

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Active Transport
- Transport that requires proteins and energy (in the form of ATP) to move solute up its concentration gradient.

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Channel Proteins
- Forms a corridor for solutes to pass through.
- Example: Ion channels or aquaporins.

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Carrier Proteins
- Protein that alternates between shapes to move solutes.
- Example: Glucose transporter or sodium-potassium pump.

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Multidrug Resistance Transporters
Carrier proteins that can actively transport drugs out of cells.
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How can Multidrug Resistance Transporters be a problem?
Could be bad for cancer or infectious disease treatments.
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Cotransport
Active transport assisted by concentration gradient.
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4 Emergent Properties of Water
- 1. Water is cohesive
- 2. Water moderates temperature
- 3. Water expands upon freezing
- 4. Water is versatile
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Definition of Hydrophilic
Water loving, ionic or polar molecules that can be dissolved water.
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Definiton of Hydrophobic
Afraid of water, non ionic or nonpolar molecules that cannot be dissolved in water.
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2 Types of Reactions that require Enzymes:
- 1. Synthesis
- 2. Break down
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Describe a Synthesis Reaction
Dehydration: the removal of a water molecule by joining 2 polymers.
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Describe a Break Down Reaction
Hydrolysis: breaking apart two polymers by adding a water molecule.
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