Micro lab final

negative staining

background dark, cell light.  Cell repels negative charge.  Morphology. 

+ to stain cell itself, sticks to ecll wall. Thick PGW is positive, thin PGW, thick LPS is negative. 

nearly pointless unless dealing with bacillus.  Add steam to cause panic/formation.  Malachite green for spores, saffranin for vegetative cells

endospores in endospore stain

2nd stain in gram, second stain in endospore

glucose, sucrose and lactose with a pH indicater

monosaccharide good for differentiating.  Simple. 

disaccharide. 

fermented by enteric organisms, usually

citrate salts as carbon source.  utilize difficult environment and break down salts (increase in +ions makes acidic color change

hyrdolyze starch or turn black with iodine.  Usually means it's living in the earlier part of the digestive tract

protein (casein) hydrolysis. 

lipid hydrolysis (creates lipidase). 

chooses one--qualitative.  Yes or NO

Selective media for gram +.  Broth is better than plate.  Kills gram - with alcohol

Selective for gram -, differential for lactose fermentation (green metalic ++, purple +, no color no lactose ferm). 

Quantitative.  Yes or no, but also how much.

like EMB but contains bile salts.  Differential for lactose fermentation (purple) but also selective for gram - and selective for enterics. 

mannitol salt agar.  Has high levels of salt, so selevtive for facultative halophiles.  Differentiates for mannitol (7-C alcohol) fermentation. 

• alpha: "incomplete".  Alters RBCs
• beta: complete destruction of RBCs
• gamma: no hemolysis

can it turn H2O2 into O2?  Protect itself.  Make catalase.