.
This type of microdcopy uses filters thst inhance the contrast of light waves that are in different phase due to passing through substances of vsrius densities. This is used when the speciman is not going to be stained
phase contrast
What arre the movements of the bacteria?
runs and tumbles
this type of moement will appear as bacteria wobbling as they move in a linear direction
runs
this type of movement that most often appears as a single sell rotatioing like a propeller
tumbles
All small cells and molecules in solution will exhibit a random shaking/twitching type of movement called
brownia motion
What is the tppe of motile is expressed in the above picture
left picture- non-motile species
right picture-motile species
This is a particular species that was obtained froma clinical speciman collection and isolated from other resident bacteria present in the specima.
a clinical isolate
Are their many specoes of harmless resident flora present
yes
these components are toxic to normal flora and they aid in the isolation and subsequent indentification of the pathogen. In this, only certain bacterial species aare "selceted"
selected components
What relys on the use of a pH indicator that will result in acolor change specific to the individule species
differential media
this is a molecule that changes color depending on the pH that it is exposed to
pH indicator
If the substrate is utilized by the isolate, the pH will do what, and this results in what?
decrease; a change in a color of the pH indicator
What is the result of the pH indicator being introduced to the chemical
the area surronding the colony or the colony itself will change color
Is mannitol salt algar selective component or differential media
both
What is mannitol salt algar used to do
isolate and identify pathogenic bacterium, Staphococcus aureus in clinical speciman
What makes Mannitol Salt Algar to be so selective? Why?
a high salt consintration; most microbes do not like the high salt consintration
What is more sensitive to salt; gram positive or gram negative
gram negative
What is the pH indicator in the MSA
phenol red
What components allow MSA to be differential
mannitol and phenol red
Gram positive bacteria id only able to metabolize mennitol. S aureus is gram positive. What happens when it is put into the mannitol salt algar
the pH is decreased causing the pink/red color to turn to yellow
The biochemical process of generating energy from a carbon source
metabolism
Wha do heteratrophes utilize to generate energy
organic carbon in the form of carbohydreates(sugars)