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The name for the equation that relates resolution to wavelength
Abbes' Equation
: smaller wavelength means smaller (better) resolution
d=0.621(wavelength)/n sin a
TEM
Transmission Electron Microscope
Tunneling Electron Microscope
Can a TEM produce a 3D image?
No
What is one main advantage of an SEM over a TEM?
The SEM can produce 3D images
Give 3 reasons to keep the column/sample chamber under vacuum
Increases the 'mean free path' of the electrons
The column stays cleaner
Prevents oxidation of the W filament
What can happen to cells in a vacuum?
They can rupture
Name 2 alternatives for solving the problem of cells rupturing in a vacuum
1. Use a partial vacuum (requires less preparation, but the quality of the images will not be as good)
2. Preserve the sample and use critical point drying
What type of metal is the filament made from?
Tungsten
Why should you set the filament temperature to the point of most effective saturation?
Because this will emit the most electrons without overheating the filament and breaking it down
The average distance an electron can travel before hitting something
Mean Free Path
Name the 2 types of lenses in the SEM
Condenser Lense
Objective Lense
Which lense focuses electrons on the sample?
The objective lense
Which lense controls the intensity (number of electrons) in the beam?
The Condenser Lense
A ___ aperature size gives better resolution and depth of field
smaller
A __ scan spped gives better quality of image
slow
The mean free path must be ___ than the optical column
longer
Name the two types of pumps that make up the vacuum system. Which is more expensive?
Mechanical Pump
Diffusion Pump (more expensive)
The term for the rotating stage
Goniometer
Name the 3 types of electron/sample interactions we focus on
Primary backscatter
Secondary electrons
X rays
Which type of interactions allow us to get topographic (surface) information?
Primary backscatter
Secondary electrons
Which type of electron/sample interaction has elastic collisions?
Primary backscatter
Which type ofelectron/sample interaction has inelastic collisions
Secondary electrons
Which type of electron/sample interaction allows us to get elemental composition information
X rays
SE
Secondary Electrons
BSE
Back Scatter Electrons
Amalgam
An alloy of mercury with another metal
Which detectors are retractable?
BSE
EDS
Which detector gives clearer 2D images? (BSE or SE)
BSE
Which detector gives good 3D images?
SE (secondary electron detector)
EDS
Energy Dispersive Spectroscopy
Does EDS rely of X-ray fluorescene or X-ray diffraction?
Fluorescence
Increased accelerative voltage generally gives ____ resolution
increased
Atomic number contrast is associated with which detector?
BSE
In atomic number contrast, areas with high average atomic number appear ___ while areas with low average atomic number appear ___
Brighter
darker
Where are the scanning coils located?
In the optical column
X ray microanalysis is another name for
EDS
Author
saitclt
ID
184108
Card Set
SEM Review
Description
Review for the SEM theory section
Updated
2012-12-08T23:07:56Z
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