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Emzyme Lab Only
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What are most enzymes made up of?
proteins
pH is a measure of what?
H⁺ in solution
How does salt affect an enzyme?
Like pH there is an optimal salt level
The enzyme catecholase catalyzes a reaction where ___, the substrate becomes ___ (product).
Catechol
Benzoquinone
What does benzoquinone look like?
Reddish-brown product
What instrument can we use to measure how much color change happens during a reaction?
Spectrophotometer
How does a Spectronic 20 measure catecholase activity?
By measuring color change in reaction mixtures
How does Spectronic 20 measure color changes?
By shining light thru reactants in a test tube & measuring mat of light that penetrates thru the tube
What is transmission on the spectrophotometer?
How much light penetrates the tube
What is absorption when speaking of the spectrophotometer?
How much light is being absorbed by the sample
WHat is the relationship between product formation & absorbance?
As absorbance ↑ so does product formed
Author
medic11
ID
181887
Card Set
Emzyme Lab Only
Description
Enzyme lab for 11-6
Updated
2012-11-05T22:06:00Z
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