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Emzyme & Photosynthesis Lab
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What are most enzymes made up of?
proteins
pH is a measure of what?
H⁺ in solution
How does salt affect an enzyme?
Like pH there is an optimal salt level
The enzyme catecholase catalyzes a reaction where ___, the substrate becomes ___ (product).
Catechol
Benzoquinone
What does benzoquinone look like?
Reddish-brown product
What instrument can we use to measure how much color change happens during a reaction?
Spectrophotometer
How does a Spectronic 20 measure catecholase activity?
By measuring color change in reaction mixtures
How does Spectronic 20 measure color changes?
By shining light thru reactants in a test tube & measuring mat of light that penetrates thru the tube
What is transmission on the spectrophotometer?
How much light penetrates the tube
What is absorption when speaking of the spectrophotometer?
How much light is being absorbed by the sample
WHat is the relationship between product formation & absorbance?
As absorbance ↑ so does product formed
What pigment causes leaves to be green?
Chlorophyll
What is the biochemical process where plants absorb light & convert it to useable chemical bond energy?
Photosynthesis
Which color in the visible light spectrum has the longest wavelength?
Red
Which color in the visible light spectrum has the shortest wave length?
Violet
The green pigment that absorbs light & is directly involved in photosynthesis is called ____.
Chlorophyll a
What colors of light are readily absorbed by chlorophyll a?
Violet/Blue & Red
Chlorophyll looks ___ ___ to the eye.
Bluish Green
___ ___ also absorb solar energy but they then pass this absorbed energy over to chlorophyll a for photosynthesis.
Accessory Pigments
What color does chlorophyll b look to our eyes?
Yellow-Green
____ absorb a great deal in the blue & green range & appear to our eyes as various shades of yellow or yellow-orange.
Carotenoids
A ___ ___ layer on a leaf prevents water loss & but also prevents gas exchange.
Waxy Cuticle
The outer layer of cells on a leaf contain ____ through which gas exchange occurs.
Stomata
What is the function of air spaces within a leaf?
Allow diffusion of gasses throughout interior of the leaf to reach all cells
Name 2 functions vascular tissue within leaves.
Water & minerals to leaf cells
Conduct excess energy rich sugar to other plant parts for storage
Stored glucose molecules are chemically bonded together for later use & stored as ____.
Starch
What method can be used to separate components of a mixture of molecules?
Paper Chromatography
What 2 things determine rate at which solvent travels up paper in paper chromatography?
Molecules affinity for the paper
Molecules solubility in solvent
A completed paper chromatography strip is call a ____.
Chromatogram
What is happening to a leaf when it is senescing?
It is declining & dying
Plants that drop their leaves at the beginning of the inhospitable season are called what?
Deciduous
During the process of senescence a plant does what?
Strips anything it can fr the leaf & stores it in preparation for dropping the leaf
Author
medic11
ID
181549
Card Set
Emzyme & Photosynthesis Lab
Description
Emzyme & Photosynthesis Lab
Updated
2012-11-04T14:07:12Z
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