Lymphoid Tissue Cytology

  1. Where is lymphoid tissue found?
    found thoughout the body in nodes, tonsils, and intestines
  2. What is the function of lymphoid tissue?
    filter blood and lymph
  3. What does lymphoid tissue function as?
    • humoral immunity
    • responds to many insult in the body
  4. What is lymphadenopathy?
    • enlarged lymph node
    • lymph gland disease
  5. When do lymph nodes enlarge?
    • primary or systemic disease
    • inflammation
    • hyperplasia
    • first degree neoplasia and second degree neoplasia
  6. Which lymph nodes do we palpate?
    • submandibular
    • axillary
    • prescapular
    • inguinal
    • popliteal
    • mesenteric
  7. How do we make a node aspirate?
    • same as general cytology
    • make 2 smears
    • stain one
  8. How do we do an impression smear of a node?
    • remove node
    • cut open
    • touch slide (normal area and abnormal area)
  9. Are segs normally seen in a lymph node cytology?
  10. _____% of cells are _____.
    • 90
    • small lymphs
  11. Other than small lymphocytes, what else will we see on the cytology?
    • prolymphs
    • lymphoblasts
    • plasma cells
    • macrophages
  12. Describe small lymphocytes (size, shape, cytoplasm, nucleus, chromatin).
    • 10 u
    • round
    • thin rim of blue cytoplasm
    • round nucleus
    • clumpy chromatin
  13. Describe prolymphocytes (nucleus shape, chromatin, cytoplasm).
    • nucleus round or my be indented
    • chromatin less clumpy than lymphocyte
    • more and paler bue cytoplasm than lymphocyte
  14. Describe lymphoblasts (size, cytoplasm, nucleus, chromatin, nucleoli).
    • 15 - 25 u
    • abundant of basophilic cytoplasm
    • large nucleus
    • lacey chromatin
    • 1 - 3 nucleoli
  15. Describe a plasma cell (size, cytoplasm, nucleus, chromatin).
    • medium size
    • moderate to abundant deep blue cytoplasm
    • clumpy chromatin
    • perinuclear clear zone
  16. What do mott cells do?
    make antibodies
  17. Describe a macrophage (size, cytoplasm, nucleus)
    • large cell
    • abundant pale blue cytoplasm with vacuoles
    • pale vesicular nucleus
  18. Is an enlarged node normal or abnormal?
  19. Whast could cause an enlarged node?
    • hyperplasia
    • inflammation
    • neoplasia
  20. What types of inflammation could cause an enlarged node?
    • acute
    • eosinophilic
    • chronic
  21. What are the two types of neoplasia in a node?
    • primary
    • metastatic
  22. What does a cytology of a hyerplasia lymph node look like?
    • small lymphs (>70% seen)
    • numerous plasma cells
    • increase in prolymphs, blasts, and macrophages
    • may see mast cells, eosinophils, few sges, mott cells
    • organisms may be present
  23. Where would there be hyperplasia in a lymph node?
    immune system stimulated for some reason
  24. Do the lymphocytes look normal on a cytology of hyperplasia of a lymph node?
  25. If there are predominately segs on a cytology of lymphadenitis, then what is it?
    purulent inflammation
  26. If there are more eosinophils on a cytology of lymphadenitis, then what is the cause?
    allergy or parasite
  27. If there are more macrophages on a cytology of lymphadenitis, then what is the cause?
    • fungi
    • foreign body
  28. Where do we look for the etiologic agent (organisms) in lymphadenitis?
    • in cytoplasm of segs and macrophages
    • between cells
  29. What is another term for primary neoplasia?
  30. What species is lymphoma common in and what else do we see with the patients who have lymphoma?
    • dogs and cats
    • hypercalcemia (elevated calcium levels)
  31. If there are many lymph nodes enlarged without dermatitis then what should we suspect it is?
  32. What does a cytology look like for lymphoma?
    • monomorphic population of large lymphs
    • plasma cells are rare
    • few small lymphs
  33. What are the different types of lymphoma?
    • lymphoblastic
    • prolymphocytic
    • histiocytic
    • lymphocytic
  34. Which type of lymphoma is the most common?
  35. Which type of lymphoma is the rarest?
  36. Who determines the type of lymphoma?
  37. We don't often see "malignant criteria" for _____.
  38. What is secondary neoplasia?
  39. What does a cytology look like of metastasis?
    • large cells with neoplastic criteria
    • cells may be unrecognizable
  40. What is the difference between primary neoplasia (lymphoma) and secondary neoplasia (metastasis)?
    • primary neoplasia (lymphoma) starts in the lymph nodes
    • secondary neoplasia starts somewhere else and metastasizes to the lymph nodes
  41. What does the normal cytology of a spleen look like?
    • similar to a normal node...
    • except 75% small lymphs
    • increased prolymphs, blasts, and plasma cells
    • many rbcs, obsure cells
  42. Why would we do a cytology of the spleen?
  43. Image Upload 1
    What is this?
    acute inflammation in the lymph node
  44. Image Upload 2
    What is this?
    • blastomyces
    • organism in acute inflammation
  45. Image Upload 3
    What is this?
    • cryptococcosis
    • organism in acute inflammation
  46. Image Upload 4
    What is this?
    • histoplasma
    • organism in acute inflammation
  47. Image Upload 5
    What is this?
    hyperplasia in a lymph node
  48. Image Upload 6
    What is this?
    hyperplasia in a spleen
  49. Image Upload 7
    What is this?
    • leishmania
    • organism in acute inflammation
  50. Image Upload 8
    What are the two large cells?
  51. Image Upload 9
    What is wrong with this animal?
  52. Image Upload 10
    What is wrong with this animal?
  53. Image Upload 11
    What are the large cells?
  54. Image Upload 12
    What is this?
    mast cell tumor
  55. Image Upload 13
    What cells are these?
    Mott cells
  56. Image Upload 14
    Is this cytology of a lymph node normal or abnormal?
  57. Image Upload 15
    What cell is this?
    plasma cell
  58. Image Upload 16
    What cell is the arrow pointing to?
  59. Image Upload 17
    What type of neoplasia is this?
    secondary neoplasia
  60. Image Upload 18
    What organ is this a cytology of?
    the spleen
Card Set
Lymphoid Tissue Cytology
Lab Tech ll