Lab A&P II Dig. Physiology

Lipids are fats. The substrate is either lilk fat or litmus cream

The enzyme is lipase (pancreatic)

The monomer for absorption is fatty acids

it takes 90 minutes in the 37C bath

The reagent is Litmus (may be built into substrate)

• Color indication:
• Acidic: pink/lilac/lighter
• Basic: blue/dark purple

The substrate for the test is BAPNA

The enzyme is trypsin

The monomer for absorption is amino acids

60 minutes in 37C bath

The reagent is built in

• Color indication:
• yellow-AA
• clear-protiens

• *spot plate test
• The substrate is starch

the enzyme is amylase

the monomer for absorption is simple sugars

30 minutes in 37C bath

• reagent: Iodine
• Benedicts in boiling water

• Color Indication:
• Iodine: black with starch
• Benedicts: orange with monosaccharides

Hepatic Portal system

EXCEPT for monoglyceride and fatty accids, which goes to the lacteals