Test 2 Micro (Ppts 7-8)

  1. Newly emerged bacterial gene causing superbugs in Europe.
  2. Host Range
    Bacteria that a phage can bind to.  Never all of one species, but possibly strains from more than one species or even genus
  3. What can attach to the sex pilus of a bacteria?
  4. Which type of phage produces a clear plaque?
    Virulent phage
  5. Which type of phage produces a cloudy plaque?
    Temperate phage
  6. Which experiment proved DNA as genetic material?
    • Hershey chase experiment.
    • DNA labeled with 32P, phage labeled with 35S.
    • 32P seen in bacteria.
  7. Restriction endonuclease
    Destroys any DNA that is not methylated in a bacterium.
  8. Identify strains by their susceptibility to a set of phage.
    Phage typing
  9. What type of genes does a conjugal plasmid carry?
    • Replicatoin
    • Phenotype
    • Transfer
  10. What type of genes do non-conjugal plasmids carry?
    • Replication
    • Phenotype
  11. How many origins of replication does a conjugal plasmid have?  What are they?
    • Transfer origin (makes 1x DNA for transfer by conjugation)
    • Vegtative origin (makes 2x DNA, not related to conjugation)
  12. When conjugal plasmids get inserted into a bacterial chromosome, this causes a piece of the bacterial chromosome to be transferred, but not generally the plasmid.  Is an example of?
    High Frequency Recombination (doesn't change female into male)
  13. Bacterial Chromosome Map is calibrated in what units?
    Minutes of mating
  14. What is Cosmid Cloning?
    Cosmid has plasmid replication site and a "cos" obtained from DNA phage.  Causes bacteria to circularize and be packed into phage particle.  Transfers big pieces of DNA.
  15. Why arent' all bacteria males?
    Phage and detergents kill males
  16. What favors "maleness" in bacteria?
    Antibiotics, Heavy metals
  17. What is the 4th leading cause of death in the US?
    Nosocomial infections
  18. The complete destruction of all living forms of life.  Includes bacteria, spores, fungi, and viruses.
  19. The use of agent to lower the number of growing microorganisms.  Does not provide sterility, as spores and some viruses may survive.
  20. An agent which lowers the count of viable microorganisms; usually applied to inanimate objects.
  21. An agent which lowers the count of viable microorganisms; usually applied to paitients.
  22. Critical items should be _______
    Semi-critical require _________
    Non-critical items require ________
    • Critical = sterilization
    • Semi-critical = high-level disinfection
    • Non-critical = low-level disinfection
  23. Agents which inhibit the growth of microorganisms; upon removal of the agent, growth may resume.
  24. Agents which kill microorganisms; upon removal of the agent, growth cannot resume.
  25. The irreversible inability of a microorganism to reproduce.
    Microbial death
  26. Killing of microorganisms follows a log function when relatively high concentrations of an agent are used.  What shape does the curve take at lower concentrations of agent?
  27. Enveloped spores are most resistant to germicidal chemicals.  What is least resistant?
    Enveloped viruses (host cell membranes): Herpes, heptatitis, HIV
  28. Alcohol is most active at what percent?
  29. Agents that interfere with membrane function?
    • SOAP:
    • Surface-active agents
    • Organic solvents
    • Alcohols (50-70%)
    • Phenols
  30. Agents that denature proteins?
    • Acids and alkalis
    • Phenols
    • Acetone and other organic solvents
    • Alcohols
  31. Agents that destroy or modify the functional groups of proteins.
    • Heavy metals
    • Oxidizing agents
    • Dyes
    • Alkylating agents
  32. Active ingredient in purell?
    Ethyl alcohol
  33. What enzyme would make the bacteria explode?
  34. Ortho-phthaladehyde (OPA)
    Does it require activation?
    Does it irritate eyes or nose?
    What pH's is it stable at?
    Does it require exposure monitoring?
    Does it have an odor?
    • No
    • No
    • Stable over a wide pH range
    • No
    • No
  35. Has the quickest sterility time against Mycobacterium bovis?
  36. Found in personal care and consumer products.  Bacteriostatic against gram-negatives and gram positives.
  37. Antiseptic with anti-bacterial, some anti-viral activity
    Effective agent for the prevention and tx of oral disease
    Adsorbs negatively charged surfaces in the mouth: prolonged antimicrobial activity
  38. Explosion potential
    Ethylene oxide
  39. Has been used to sterilize buildings contaminated w/ anthrax spores.
    Chlorine dioxide
  40. Has been used to disinfect hospitals undergoing outbreaks of carbapenem-resistant Klebsiella pneumoniae
    Hydrogen peroxide gas plasma
  41. Causes single- or double- strand breaks in nucleic acids and free-radicals
    Spores generally resistant
    Deinococcus radiodurans is the most resistant organism known, has an efficient DNA repair process
    Prions are highly resistant
  42. Biologic Indicators:
    What changes media color to yellow?
    Most reliable method of monitoring sterilization.
    Germinating spores
  43. Which indicator has a dye that changes according to temperature?
    Process indicators
  44. Which indicator has a dye that changes according to temperature and time of exposure?
    Dosage indicators
Card Set
Test 2 Micro (Ppts 7-8)
Applied Bacterial Genetics, Sterilization and Disinfectants