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- plasmodium
- hemoproteus
- leukocytozoan
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What is Leukocytozoan?
a protozoan parasite commonly found in the blood of wild birds
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What is plasmodium?
parasite that is pathogenic and are responsible for malaria
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What are the clinical signs of plasmodium?
- anemia
- anorexia
- depression
- acute death
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Where can the developmental stages of plasmodium be found?
within erythrocytes, thrombocytes, leukocytes
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What are in the RBCs of an animal with plasmodium?
gametocytes
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In addition to pigment-bearing gametocytes, both _____ and _____ may be observed in peripheral blood erythrocytes in a bird with plasmodium
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What is Hemoproteus?
protozaon blood parasites common in wild birds, pathogenicity is usually low
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Does the Hemoproteus gametocytes ever push the host cell nucleus to the cell margin?
no
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What are fused ("melted") heterophil granules?
- the granules of both heterophils and eosinophils can be affected by both heparin and the staining process. they may appear poorly stained partially dissolved or cused in some cases
- this is a common artifact that cannot always be avoided
- cell determination must be made by the appearance of the nucleus if possible and the color of the fused granules in the cytoplasm
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What does an avian heterophil look like?
- cytoplasm of normal, mature heterophils appears colorless and contains rod shaped granules that stain brick red
- the nucleus of the normal mature heterophil is lobed, with coarse, clumped, purple-staining chromatin
- the nucleus is often partially hidden by the cytoplasmic granules
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What does an avian eosinophil look like?
- most avian eosinophils are similar in shape to the heterophils
- the nucleus is lobed and usually stains darker than the heterophil
- the cytoplasm contains round, strongly eosinophilic granules
- granules of the eosinophil often lack the central refractile body noted in many avain heterophils
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How do we calculate the avian mononuclear cell count?
- (% lymphs + monocytes/% heteros + eos + baso) x granulocyte count from hemocytometer
- add granulocyte count to mononuclear cell count
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avian thrombocytes (platelets)
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