Reptilian Hematology

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  1. Reptile cells readily _____.
  2. Which WBCs are rare in snakes?
    • eosinophils
    • monocytes
  3. Do we know alot about reptile hematology?
  4. Where are the places we can collect blood in snakes?
    • ventral coccygeal
    • heart
    • palantine veins
  5. What is the ventral coccygeal vein?
    tail vein
  6. Do we use the palantine veins often?  Where are they?
    • no
    • roof of mouth
  7. Where is the ventral coccygeal vein?
    behind the vent
  8. What is the vent?
    scale that covers the cloaca (where poop, pee, and eggs come out)
  9. Where can we collect turtle blood?
    • jugular vein
    • occipital plexus
    • brachial vein
    • subcarapacial sinus
  10. Which place is the easiest to collect in a turtle?
  11. Where is the occipital plexus?
    back of the head
  12. Where is the subcarapacial sinus?
    under the shell right below the carapace
  13. Which turtles do we use the subcarapacial sinus the most in?
    aquatic turtles
  14. If we aren't careful what might contaminate our subcarapacial sinus sample?
    might accidently hit the lymph sinus and our lymphocytes will be elevated...the blood will be a milky color
  15. What are the places to collect blood in a lizard?
    • caudal tail vein
    • ventral abdominal vein along midline
  16. Which place is the easiest to collect blood in a lizard?
    caudal tail vein
  17. What do erythrocytes look like in reptiles?
    nucleated, pink cytoplasm
  18. What is a normal PCV for reptiles?
    20 - 40%
  19. What can cause the PCV to vary?
    • nutrition
    • sex
    • environment
  20. What do heterophils look like in reptile blood?
    numerous fusiform (rod shaped) eosinophilic granules
  21. What is the most common granulocyte?
  22. What percent of leukocytes are heterophils in reptiles?
  23. What kind of nucleus do heterophils have in reptiles?
    • single nucleus
    • round to oval
  24. What do eosinophils look like in reptiles?
    • cytoplasmic eosinophilic granules
    • nuclei single or bi-lobed (eccentric)
  25. What colors are the granules stained in eosinophils in iguanas?
    stain blue
  26. What do basophils look like in reptiles?
    blue to purple granules in cytoplasm
  27. What color are the granules in basophils in iguanas?
  28. What do the granules usually do to the nucleus in a basophil?
    obscure the basophil
  29. How many basophils do we normally see in reptiles?
    up to 40%
  30. What do lymphocytes look like in reptiles?
    • round cell with round nucleus
    • blue cytoplasm
  31. What do monocytes look like in reptiles?
    • light blue gray cytoplaasm
    • vacuoles
    • single nucleus oval to indented
  32. What are azurophils in reptiles?
    cytoplasm has fine eosinophilic granules and appears red
  33. What are azurophils derived from?
  34. How do we count azurophils?
    count it as a monocyte for our calculations but count separately on the differential
  35. What do thrombocytes look like in reptiles?
    • elliptical to round cells
    • clear cytoplasm
    • dense nucleus
  36. Image Upload 1
    Name these WBCs.  What animal?
    • heterophil on the left, eosinophil on the right
    • Aldabra tortoise
  37. Image Upload 2
    What WBC is this?
  38. Image Upload 3
    What WBC is in the middle?
  39. Image Upload 4
    What are these WBCs and what animal?
    • eosinophil on the bottom
    • heterophil on the top
    • box turtle
  40. Image Upload 5
    What is this?  What animal?
    • heterophil
    • box turtle
  41. Image Upload 6
    What is this?  What animal?
    • degranulating heterophil - vacuoles caused by stain artifacts
    • crocodile
  42. Image Upload 7
    Name these cells.
    • eosinophil on the top left
    • monocyte on the bottom left
    • lymphocyte on the right
  43. Image Upload 8
    What are these cells?
    • erythrocytes
    • erythroblasts
  44. Image Upload 9
    What are these blood parasites?
    • haemogregarines on the left
    • haemoproteus on the right
  45. Image Upload 10
    What blood parasite is this?
  46. Image Upload 11
    What WBC is this?  What animal?
    • basophil
    • iguana
  47. Image Upload 12
    What WBC is this?  What animal?
    • eosinophil
    • iguana
  48. Image Upload 13
    What WBC is this?  What animal?
    • heterophil
    • iguana
  49. Image Upload 14
    What WBC is this?  What animal?
    • monocyte
    • iguana
  50. Image Upload 15
    What WBC is this?  What animal?
    • thrombocyte
    • iguana
  51. Image Upload 16
    What is going on in this cell?  What animal is it?
    • mitosis
    • lizard
  52. Image Upload 17
    What are these two cells?
    • lymphocyte to the left
    • immature RBC to the right
  53. Image Upload 18
    What WBCs are these?
  54. Image Upload 19
    What WBC is this?  What animal?
    • heterophil
    • snapping turtle
  55. Image Upload 20
    What is this?
  56. Image Upload 21
    What WBC is this?  What animal?
    • heterophil
    • python
  57. Image Upload 22
    What are these cells?  What animal?
    • toxic heterophils
    • skink
  58. Image Upload 23
    What WBC is this?  What animal?
    • azurophil
    • snake
  59. Image Upload 24
    What WBC is this?  What animal?
    • basophil
    • snake
  60. Image Upload 25
    What are these cells from left to right?  What animal?
    • lymphocyte, eosinophilk, monocyte
    • snake
  61. Image Upload 26
    What WBC is this?  What animal?
    • eosinophil
    • snapper
  62. Image Upload 27
    Name these cells from top to bottom.  What animal?
    • heterophil, erythrocyte, lymphocyte, eosinophil
    • snapping turtle
  63. Image Upload 28
    What WBCs are these?
Card Set
Reptilian Hematology
Lab Tech ll
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