Fluid Thioglycolate Medium

FTM: yeast extract, pancreatic digest of casein, dextrose, sodium chloride, sodium thioglycollate, L-cystine, agar, resazurin, water.

Used to determine microbial growth. To test the aerotolerance of bacteria.

cultivation of strict anaerobes & microaerophiles.

Take bacteria on loop and go down to the bottom in a swirling motion.

• OBLIGATE AEROBE: require oxygen - grow at top.
• OBLIGATE ANAEROBE: no oxygen - grow at bottom.
• MICROPHILE: tolerates oxygen in small amt. - grow upper middle.
• FACULATIVE ANAEROBE: doesn't need oxygen but can use it if needed - grows bottom to middle.
• AEROTOLERANT ANAEROBE: not harmed by oxygen - grows throughout medium.

• C. sporagenes
• S. aureus