Streak Plate Method of Isolation

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  1. Why do we perform isolation streaking?
    To produce individual colonies - then transfer to sterile medium to start a pure culture.
  2. Describe technique used for isolation streaking.
    • # of streaks: Quad streak - 4
    • where on argar plate: 90 degrees 3 times and then up the middle
    • tool used: inoculating loop
  3. What is CFU & why do we use this term in lab?
    CFU: term used to define cells that produce a colony when transferred to plated media.

    ?:
  4. What would be a situation we wouldn't need to use isolation streaking?
    If it was already a pure culture.
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17147
Card Set
Streak Plate Method of Isolation
Description
microbiology
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