wet mount hanging drop slides

To view living cells to determine motility. See size, arrangements & shapes.

WET: Add drop of water on slide with loop. Inoculate bacteria in water. Add cover glass.

HANGING: Apply petroleum jelly around well of depression on slide. Apply drop of water to cover glass. Inoculate bacteria in the drop of water. Invert depression slide over cover glass.

Hanging drop allows longer viewing.

False +: Browning motion is not true motility b/c collision of water molecules. (looks like static on tv)

False -:

Observe on microscope under high dry or oil immersion