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study of cells, tissue, and organs at the microscopic level
What are the different ways to process tissue?
fix: crosslink protein and maintain tissue architecture
: remove water
embed: infiltrate/embed tissue with hard material that can be cut into thin slices
section: cut thin slices of embedded tissue
: either in light microscopy or electron microscopy
What are the different kinds of staining and their uses?
histochemical for reactive chemical groups such as charge (i.e. Hematoxylin and Eosin = H&E);
immunocytochemical (i.e. with antibodies for specific antigens)
osmium for transmission E.M. = ultra-thin sections of detailed subcellular structure
gold/metal coating for scanning E.M. = 3D-like image
a group of similar cells and surrounding extracellular matrix and extracellular fluid
What are the four major basic tissue types?
groups of tissues that act together to carryout specific bodily functions
ANAT 390 Lecture 1