anat1.txt

study of cells, tissue, and organs at the microscopic level

• fix: crosslink protein and maintain tissue architecture
• dehydrate: remove water
• embed: infiltrate/embed tissue with hard material that can be cut into thin slices
• section: cut thin slices of embedded tissue
• stain: either in light microscopy or electron microscopy

• light microscopy:
• histochemical for reactive chemical groups such as charge (i.e. Hematoxylin and Eosin = H&E);
• immunocytochemical (i.e. with antibodies for specific antigens)

• electron microscopy:
•  osmium for transmission E.M. = ultra-thin sections of detailed subcellular structure
• gold/metal coating for scanning E.M. = 3D-like image

a group of similar cells and surrounding extracellular matrix and extracellular fluid

• epithelia
• connective tissue
• muscle
• nervous tissue

groups of tissues that act together to carryout specific bodily functions