Intro to Histo.

  1. Eosin vs. Hematoxylin?
    Eosin for basic or acidophilic substances (proteins)

    Hematoxylin is for acidic or basophilic substances (DNA)
  2. What is Periodic acid-Schiff used for?
    PAS (or HIO4) turns carbohydrates into aldehydes which can be tagged purple schiff reagent
  3. What is the purpose of enzyme histochemistry?
    Soluble, non-colored substrate are converted to non-soluble, colored products via enzyme activity.

    Thus, the quantity of precipitate is proportional to enzyme activity
Author
sbandzar
ID
170244
Card Set
Intro to Histo.
Description
med school
Updated