1. measuring levels of analytes and comparing to reference values
2. observing and detecting abnormal cells under the microscope
3. detecting the presence of pathogenic microorganisms
types of laboratories
-- reference or referral laboratories: clinical lab run by pathologists
-- hospitallaboratories: bedside test ( POCT)
-- ambulatory care laboratories: CLIA-waived test
types of medical laboratories
. specimen collection and processing
. hematology department
. chemistry department
. immunology/ serology and immunohematology
. microbiology department
. pathology department
. toxicology department
purposes for laboratory testing
1. to screen patients for possible disorders
2. to establish a diagnosis
3. to monitor the patient's condition or treatment
common CLIA waived test
- urinalysis and pregnancy testing
- hematology test: hemoglobin, hematocrit
- coagulation
- chemistry: glucose, lipids
required characteristics of a laboratory professional
1. professionalism
2. interpersonal communication skills
3. observant
4. problem slover
5. organized
Preanalytical
- test are ordered
- requisition filled out
-specimen collected and labeled
-specimen processed and transported to office laboratory
Analytical
- in office lab tests performed and patient test results logged and charted
- results are logged and placed into patient charts
- results are given to physician
Postanalytical
- dispose specimens
- patient test results are interpreted and signed by physician
- patient is notified and it is recorded in chart
- final signed chart is filled
Standard precautions
are recommendations for infection control of communicable diseases
Personal protective equipment (PPE)
- goggles
- face shield
- gown
- gloves
- plexiglas shield
wearing gloves still requires
washing of the hands
Universal precautions
The assumption that blood or body fluid containing blood from any patient or test kit could be infectious.
Diseases caused by bloodborne pathogens
HIV- no vaccine
HBV- vaccine available
HCV- no vaccine
HIV is tramsmitted by
blood , semem and amniotic fluid.
HIV is a RETROVIRUS it attacks the immune system by destroying the white blood cells known as CD4+ T lymphocytes.
4 stages of HIV
1. An acute stage flu like symptoms with swallon glands
2. an asymptomatic stage no symptoms but the person is still infectious
3. Symptomatic stage appearance of opportunistic infections
4. AIDs
Hepatits B virus (HBV)
- is the most prevalent bloodborne virus.
- should be avaliable to all employees. new employees should begin vaccination within the first 2 weeks of employment. 10 days to decline have to sign a declination letter.
HAV- Hepatisis A
- not bloodborne
- attacks the liver
- trasmitted by direct or indirect contact through gastrointestional tract
- patients are placed on isolation
HCV - Hepatitis C
attacks the liver
OSHA biohazard training: OSHA requires employess to keep workers informed and protected by supplying
1. safety training
2. proper apparel (PPE)
3. a safe work environment
the average health care worker is more likely to contract
hepatisits B or hepatisits C than HIV
Engineering controls
efforts to isolate and remove pathogenic dangers from the workplace.
work practice controls
actions in a laboratory to protect workers from exposure to pathogens in blood or body fluids no
no eating
no drinking
no smoking
no applying of make-up
procedure after exposure to blood
- wash the exposed area immediately
- report the exposed area immediately to a supervisor
- file an incident report
- seek medical attention
color code blue
health hazard
color code yellow
reactivity ( instability)
color code red
flammability (fire hazard)
color code white
other such as PPE
reservoir host
an infected person who is carrying an infectious agent
portal of exit
a means of leavingthe infected host's body. through mouth , broken skin , rectum or body fluids
transmission
a means of transporting itself from the infected individual to another person by direct or indirect contact.
portal of entry
a body opening or break in the skin in another person
susceptible host
an individual who is unable to protect himself against the infectious agent