# Enzymes.txt

 .remove_background_ad { border: 1px solid #555555; padding: .75em; margin: .75em; background-color: #e7e7e7; } .rmbg_image { max-height: 80px; } author "Joshua Ellis" tags "HUCM" description "" fileName "Enzymes" freezingBlueDBID -1.0What is the Michaelis -Menten Equation Vo= Vmax[S]/(Km+[S] Vo= initial velocity Vmax= maximal velocityKm= Michaelis constant = (k-1 + k2)/k1[S]=substrate concentration What is Km? Relate it to Vmax. Michaelis constant (k-1 +k2)/k1 (all the rate constants below) k1 k2E+S <---> ES ---> E+P k-1Does not change with concentration of enzyme. Basically equal to reaction velocity = 1/2 Vmax What does a low and high Michaelis constant mean? Small Km = high affinity of the enzyme for substrate. (low concentration of substrate need to half saturate the enzyme--reach v=1/2vmax)Large Km = low affinity. Cuz a high concentration is required. What is the relationship to enzyme concentration and velocity of reaction? ALWAYS directly proportional. If you half [E] then the Vo and Vmax will also be halved How can you determine the order of the reaction? When [S] is much less than Km velocity is proportional to [S]-----FIRST ORDERWhen [S] is much greater than Km velocity is constant and equal to Vmax (independent of [S])----- ZERO ORDER How do you make the Lineweaver-Burke Plot? What does it tell you? What is the equation? Its the reciprocal of the Michaelis Menten equation It can tell you the Km and Vmax (as x and y intercepts) 1/vo= Km/(Vmax[S]) + 1/Vmax Reversible inhibitors have what type of bond? Non-Covalent What is competitive inhibition? What is the effects on Vmax, Km, and Lineweaver Burke Plot Binds to active site Vmax is the sameIncreased Km because need more [S]Graph y intersect is same but x intercept is increased (obvious slope change) What is non-competitive inhibition? What is the effects on Vmax, Km, and Lineweaver Burke Plot non active site binding Vmax decreasedKm is the same The graph x intercept is the same AuthorAnonymous ID164974 Card SetEnzymes.txt Descriptionenzymes from book Updated2012-08-04T18:48:45Z Show Answers