clin chem 5

measures of concentration of abs in blood

agglutination=antigen binding to antibodies

lab technique used to measure concentration of a solution by the intensity of its color

• serum+reagent---->color change
• the more intense the color the greater the concentration
• Visual colorimetery ex: U/A dipstick

Beers Law: the concentration of a solution is directly proportional to the amount of light absored by that solution and is indirectly proportional th the amount of lighted transmitted thru the solution

4 cuvettes

• contains reagent only
• used to zero  the machine to 100% transmittance and 0% absorbance

standard solution only (you must buy the solution)

reagent+control solution (must buy)

reagent+serum

• A: St. Conc/St. abs=Pt. Conc/ PT. abs
• B: St. Conc/St. abs=Cont. conc/cont. abs
• is the test valid?

C. concentration curve

• Vet test^tm
• measure light reflected of reagent pads then calculates concentration
• includes norm ranges
• serum or lithium heparin plasma

• count 5 of the 25 squares that are divided into 16 tiny squares and count those individual squares
• If touching top or right lines, COUNT
• If touching bottom or left lines, SKIP