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lecture 9
enumeration of growth
period of time with little growth where bacterium adopt to new conditions
lag phase
period of extreme growth and lots of binary fission
exponential phase
time when nutrients run out, toxin are produced, or crowding occurs when bacteria feed off dying bacteria
stationary phase
period where the overall number of bacteria decrease
death phase
number of bacteria growing per hour
growth rate constant
what is the formula for generation time and what does each mean respectively N=Ne^(ut)
final cells = one cell * growth rate * time
growth factors bacterium produce during exponential phase such as acid, gas and ethanol
primary metaboblite
growth factors bacterium produce during stationary phase to compete with other bacteria such as antibiotics and bacterioins
secondary metabolite
cells well adapted to get nutrients from other cells during the death phase
persister cells
culture in a flask which gives all nutrients in the beginning and waste builds up
batch culture
using a chemostat nutrients and waste controlled to determine growth rate
continuous culture
apparatus used for a continuous culture
chemostat
counts all cells alive and dead
direct cell count
grid which allows for counts of immotile cultures; not useful for large numbers
petroff hauser chamber
electrode measures interruption of electric current as cells pass through a counter; not good for soil samples
coulter counter
count only live cells in colonies/mL
viable cell count
spread diluted sample over agar for a viable cell count of colonies
spread plate
pour diluted culture within agar for viable cell count
pour plate
large volumes can be counted on filter paper
membrane filter
fifteen test tubes with different dilutions are used to determin probability of growth
most probably number
how cloudy a solution of bacteria is based on measurements of spectrometer; limited to dense bacterium
turbidity
used to measure acid from fermentation of bacterium
biochip
acid from fermentation conducts elections which results in __
conductivity
upside down test tube used to collect gas bubbles to measure bacterium
durham tube
enzyme used to nonquantatively tell if live cells remain in a culture by reacting with ATP to produce light
luciferase
Author
epaynich
ID
16462
Card Set
lecture 9.txt
Description
Micro 221 lecture 9
Updated
2010-04-27T19:27:33Z
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