Aminoacyl-tRNA synthetaase is a molecule that catalyzes the binding of an amino acid to the correct tRNA molecule. To which group of macromolecules does aminoacyl-tRNA synthetase belong?
Proteins
The position of carbons in the pentose sugar, deosyribose, is what makes DNA strands ___________.
Antiparallel
True or False: An RNA molecule is produced in the nucleus.
True
What would be the sequence of nucleotides in the newly synthesized RNA strand given this DNA strand template: 3' ACGCATGCGTACCAT 5'
3' AUGGUACGCAUGCGU 5'
True or False: Temporal isolation is considered a postzygotic barrier.
False
How are nucleotides added to a new strand of RNA?
An RNA polymerase
An anabolic pathway would result in the synthesis of a _____________.
macromolecule
What is the sequence of amino acids in the polypeptide encoded by this gene: 3' TAC ATA GGA CTG CCG 5'
MET-TYR-PRO-ASP-GLY
In a species of yeast, the tRNA population does not contain any tRNAS with the anticodon 3'ACC 5'. During translation, these yeasts could not produce any polypeptides that contain the amino acid:
Trp
The reduction of _______________ has a positive delta G.
carbon dioxide
The walls of gram-negative bacteria have relatively small amounts of _____________.
Peptidoglycans
What type of organism would obtain energy from inorganic molecules and carbon from carbon dioxide?
Chemoautotrophic organism
______________ is the exchange of genetic material through infection by a phage.
Nondisjunction
True or False: A spontaneous reaction is a reaction that is exergonic.
True
Refers to that which can or does move matter.
energy
Energy that is associated with moving matter.
Kinetic energy
Energy that is stored.
Potential energy
All of the chemical reactions that occur in an organism.
Metabolism
_______________ is the physics of energy transofrmation that occur in a collection of matter.
Thermodynamics
The _________ law of Thermodynamics states that energy is neither created nor destroyed.
The First Law of Thermodynamics
The ___________ law of Thermodynamics deals with the ordering of matter and states that all energy-affected matter in the universe is becoming random.
The second law of thermodynamics
____________ is the disorder of matter in the universe.
Entropy (s)
The energy available (or required) to do work in a given system.
free energy (G)
The change in free energy (delta G) is ______________ if energy enters the system, and _____________ if it leaves the system.
endergonic, exergonic
An exergonic reaction is unstable, has a negative delta G, and is therefore a ___________________.
spontaneous reaction
The total energy is a system is known as _______________.
Enthalpy (H)
An ______________ reaction has a negative delta H and will release heat, whereas an ____________ reaction has a positive delta H and whill absorb heat.
exothermic, endothermic
A cell does three types of work: _____________ (contracting muscle cells), _________________ (moving substances across the cell membrane), and ___________ (non-spoontaneous reactions between molecules).
mechanical, transport, chemical
A major source of chemical energy for non-spontaneous reactions between molecules is _____________.
Adenosine triphosphate (ATP)
The use of an exergonic (energy-releasing) process to drivve an endergonic (energy-requiring) process is called _____________.
energy coupling
A ____________________ reaction is energetically unfavorable (ex. glutamic acid to glutamine).
non-spontaneous
For each piece of DNA, replication begins at numerous ____________________ which are composed by a team of enzume proteins that are involved in DNA replication.
origins of replication
_________ unwinds the DNA helix during DNA replication.
helicase
______________________ keep the DNA strands separate during DNA replication.
single-strand binding proteins
__________ initiates DNA replication.
primase
_________________ adds nucleotides to the unwound parent molecule during DNA replication.
DNA polymerase
The arrangement of DNA strands is ________________, with one strand designated the 3'to 5' strand and the other the 5' to 3' strand.
antiparallel
Nucleotide addition is a continuous process along the ____________ strand of DNA.
leading strand
The ___________ strand of DNA has a discontinuous mode of replication.
lagging strand
The point at which DNA polymerase can work on the lagging strand during replication.
replication fork (where DNA is unwinding and progressing outward until it runs into a previously synthesized fragment.)
During DNA replication, the lagging strand is started with the help of ____________ which is made of a few nucleotides of RNA and is synthesized by primase.
primer
Short stretches of DNA that are added to the primer sequence.
Okazaki fragments
This enzyme seals the DNA fragments into a continuous strand of DNA (on the lagging strand during DNA replication).
ligase
DNA is transcribed into messenger RNA, and messenger RnA is translated into proteins. This is accomplished via two processes: ___________ and ____________.
transcription and translation
The _________ code was "cracked" by Marshal Nirenberg.
genetic code
A ________ is a sequnce of bases that make up the genetic code of a molecule.
codon
During the first phase of transcriptions, one of the two strands of DNA is transcribed into a single strand of copmlementary RNA termed ________________.
messenger RNA (mRNA)
The DNA strand that is copied is known as the ______________ strand.
template
This enzyme separates the DNA strands and joins the RNA nucleotides along the exposed DNA template.
RNA polymerase
The RNA polymerase starts its process of separating the DNA strands and joining the RNA nucleotides along the exposed DNA template when certain proteins,_________________, bind to a cepcific starting point, the ____________.
transcription factors, promoter
An RNA sequence that signals the end of RNA synthesis.
terminator sequence
There are two other types of RNA needed for protein synthesis: ______________(combines with proteins to form ribosomes) and _______________ (transport amino acids to the prowing polypeptidde chain).
ribosomal RNA (rRNA), transfer RNA (tRNA)
____________ are cellular structures where polypeptides form.
Ribosomes
A sequence of three nucleotides that is complementary to a sequence of bases in the mRNA strand and is found in every tRNA molecule.
anticodon
This enzyme insures that a given tRNA molecule picks up only its particular amino acid.
aminoacyl-tRNA synthetase
To insure high fidelity of protein translation, each tRNA has a corresponding _____________.
aminoacyl-tRNA
A ___________ signals the end of translation. These include UAA, UAG, and UGA, and they do not code for any amino acids.
At the end of translation, a ________________ binds to the stop codon and the newly synthesized polypeptide is liberated from the ribosome.
release factor protein
______________ describes how genes can be "turned on" to synthesize a needed protein, or "turned off" to stop synthesis of a protein that is no longer needed.
gene regulation
Occurs when each new molecule of DNA is comprised of one parental strand and one newly synthesized strand.
Semiconservative DNA replication
____________ have a lack of a membrane-bound nucleus, have less subcellular speicialization, and are very small (often described as single-celled organisms). Shapes can be either spherical, rod-shaped, or helical.
Prokaryotes
The individual cells whose shapes are used to classify prokaryotes are: _________ (spherical), __________ (rod-shaped), __________ (helical/spirally).
coccus, bacillus, spirillum
Prokaryotic organisms can belong to the domain ___________ or the domain ___________, while those organisms that have a nucleus comprise the third domain _______________ (Eukaryotes).
Achaea, Bacteria, Eukarya
Archaea are sometimes referred to as ______________ because these organisms would inabhit extreme environments (i.e. hot springs, salt ponds, arctic ice, etc).
extremophiles
Prokaryotes replicate via __________. Cell division in which a prokaryotic chromosome replicates and the mother cell pinches in half to form two new daughter cells.
binary fission
The DNA is prokaryotes is concentrated in the _____________.
nucleoid
Prokaryotes often have smaller rings of extrachromosomal DNA termed ___________. Most of these consist only of a few genes and are not required for survival in most environments becaue the prokaryotic chromosome programs all of the cell's essential functions.
plasmids
_____________ occurs when prokaryotes acquire genes from their surrounding environment.
Transformation
Harmful strains of bacteria are known as __________ bacteria.
pathogenic
Genetic material between prokaryotes can be exchanged through ______________. Theis requires that two living prokaryotic cells physically join with one another.
Conjugation
During conjugating, DNA transfer is done when the "male" uses an appendage calld a ___________ to transfer the DNA to a "female."
pilus
The __ factor, fertility factor plasmid, is a plasmid that is used to produce a pilus which is used during conjugation.
F factor
__________________ is the exchange of DNA between prokaryotes which is made possible by phages.
Transduction
A __________ is a virus that infects bacteria.
phage
_______________________ is when the phage DNA inserts somewhat benignly into the bacterial host chromosome. It can lay dormant for many generations until it is under certain conditions when it will excise itself from the bacterial chromosome, then replicate and form new phages that burst out of the cell.
specialized transduction
These organisms obtain their energy from light and use carbon dioxide as a carbon source.
Photoautotrophs
These organisms obtain their energy from a high-energy molecule and use a complex form of carbon as a carbon source.
Chemoheterotrophs
THese organisms obtain their energy from light and use a complex form of carbon as a carbon source.
Photoheterotrophs
These organisms obtain their energy from a high-energy molecule and use a carbon dioxide as a carbon source.
Chemoautotrophs
These organisms have membrane bound nuclei.
Eukaryotes
The cells of all organisms are encased in a _______________, which consists of a phospholipid bilayer that is selectively permeable.
plasma membrane
Bacterial cell walls contain some amount of _____________, which is a polymer consisting of sugar and polypeptides.
peptidoglycan
____________ is a technique used to analyze the amount of peptidoglycan in the cell wall of an unknown organism.
Gram's stain
If an organism tests ______________ (purple) on a Gram's stain test then the organism has a lot of peptidoglycan in its cell wall.
gram-positive
If an organism tests _______________ (pink) on a Gram's stain test, then the organism has an additional outer membrane composed of lipopolysaccharides and less peptidoglycan.
gram-negative
Gram-negative bacteria have an additional outer membrane with attached _______________, making the cell's wall more complex overall.
lipopolysaccharides
Gram-negative bacteria, having an additional membrane can make them more resistant to _______________ (antibacterial medications).
antibiotics
In addition to cell walls and cell membranes, many bacteria have an additional layer outside the cell wall termed the __________.
capsule
A __________ is a long appendage specialized for locomotion.
flagellum
Bacteria with flagella alternate between tumbling and directional movement. In some cases the movement is random, but in other cases there is a net oriented movement, or __________, toward or away from a stimulus.
taxis
The state of any two species having an extended and intimate assocation is termed __________.
symbiosis
____________ (interacting organisms) have varying relationships with one another.
Symbionts
The __________ is the larger of the two interacting organisms and the ____________ is the smaller of the two interacting organisms during a symbiotic relationship.
host, microsymbiont
If two organisims derive mutual benefit from association, they are experiencing ___________ (+,+).
mutualism
If one organism derives benefit from assocation, but the other is harmed they are experiencing _____________ (-,+).
parasitism
If one organism derives benefit from association and the other organism is not affected, or neither organism derives benefit nor harm, they are experiencing ______________ (+,0) or (0,0).
commensalism
__________ are proteins secreted by prokaryotes, whereas ___________ are protein components of the outer cell membrane in some gram-negative bacteria.
exotoxins, endotoxins
Many bacteria form _____________ (thickly coated, resistant cells) that allow them to survive extreme conditions.
endospores
The formation of endospores is called _______________. This process is triggered by a decline in key nutrients in the environment surround the bacteria.
sporulation
An ____________________ may occur when bacteria is present in an environment and then the environment changes to provide an opportunity for the present bacteria to cause infection.
opportunistic infection
The utilization of various bacteria for the cleansing of toxins in the environment is known as __________.
bioremediation
______________ is the process that nitrogen is transformed into a biologically usable state by certain nitrogen-fixing bacteria.
nitrogen fixation
Organisms that live on dead and decaying matter are known as _________.
saprobes
The ________ cycle is the process by which nitrogen is made usable by nitrogen-fixing bacteria and is then reconverted by soil bacteria back into atmospheric nitrogen.
nitrogen
Plants such as peas, alfalfa, and soybeans are capable of housing nitrogen-fixing bacteria. These plants are known as ________.
legumes
The term __________ is applied to serveral species of bacteria that participate in plant-prokaryote mutualism.
rhizobia
Rhizobia, bacteria thta participate in plant-prokaryote mutualism, migrate to the plants roots an d form ___________ in the plant's roots which are favaorable for the fixation of nitrogen by the bacteria that live inside.
nodules
_____________(blue-green algae) are capable of nitrogen fixation.
Cyanobacteria
______________ is a general term that refers to any organism, environment, or cellular process that lacks or does not require oxygen, and can even be poisoned by oxygen.
Anaerobic
Organisms have various metabolic relationships with oxygen. ______________ require oxygen, ____________ have no need for oxygen, and ________________ can alternate their oxygen requirement.
The net movement of water across a selectively permeable membrane, from lower solute concentrations to higher solute concentrations is known as _____________.
osmosis
______________ is a catabolic pathway comprised of a series of steps that convert the chemical energy in glucose into the energy contained in ATP.
cellular respiration
____________ is short for theh chemical process known as "reduction-oxidation." It refers to the transfer of electrons that occurs during many chemical reactions.
redox
A molecule that gains an electron is reduced, meaning that there has been a "reduction" in its positive charge. This is called ________.
reduction
A molecule that loses an electron is oxidized, and this loss of electrons is termed ____________.
oxidation
Some molecules have a very high afinity for electons, therefore, they are more likely to oxidize other molecules. The affinity for electron sis termed_________.
electronegativity
The most prevalent electron carrier is nicotinamide adenine dinucleotide. This electron carrier can exist in its reduced form _________ or as an oxidized positive ion ___________.
NADH, NAD+
______________ is an enzyme that in conjunction with hydrogen can make NAD+ become reduced to NADH.
dehydrogenase
The protein rich, semi-fluid part of the cell in which the cell's organelles are immersed is called ___________.
cytosol
The first step in cellular respiration is called __________ which involves the initial breakdown of glucose, pyruvic acid, water, and reduced electron carriers.
Glycolysis
_________ is also known as pyruvic acid and is part of the breakdown process of glycolysis.
Pyruvate
During the second step of the ten step process of glycolysis, ____________________ involves a phosphorylated molecule transferring a phosphate group to ADP. This is the general way in which ATP is produced during glycolysis.
substrate-level phosphorylation
The process by which glucose is partially broken down and NAD+ is regenerated is ______________.
fermentation
Two common types of fermentation are _____________ and ______________.
alcohol fermentation, lactic acid fermentation
During this cycle, electrons are removed from acetyl CoA and these electrons reduce more NAD+, along with another electron carrier, FAD.
Krebs cycle
____________ known as the powerhouses of a cell, house the phase of cellular respiration in which high energy electrons, NADH and FADH2, provide energy to do work.
Mitochondrion
A set of membrane-bound enzymes in the mitochondrion are collectively referred to as the ____________.
electron transport chain
Positively charged hydrogen atoms are also known as ___________.
protons
The movement of protons across the inner mitochondrial membrane creates a ____________ that will be used to synthesize ATP.
charge differential (voltage)
When oxygen acts as a __________ acceptor, there is a maximal amount of free energy released meaning more protons can be transported, thus a greater charge buildup occurs accross the inner mitochondria membrane.
terminal electron acceptor
The ______________ matrix is the part of the mitochondrion enclosed within the inner membrane, which houses the enzymes and substrates for the Krebs cycle.
Mitochondrial matrix
_____________ occurs when ADP is phsphoylated to make ATP.
Chemiosmosis
Chemiosmosis is accomplished in the presence of the protein complex ________ synthase, which is also located in the inner mitochondrial membrane.
ATP synthase
The generation of ATP from chemiosmosis is referred to as _____________ phosphorylation because oxygen's oxidative proprty allows a large amount of free energy to be made available for ATP synthesis.
oxidative phsophorylation
___ and _________ are electron carriers involved in the Krebs cycle.