Clinical Chemistries

  1. What types of samples are used for clim chem?
    • Plasma
    • Serum
    • Whole blood
    • Urine
    • Feces 
  2. How soon should a sample be used after being collected and what should be done with the sample if it cannot be used w/in that time?
    Used w/in the hour or frozen
  3. How long should plasma and serum be centrifuged and at what rpm?
    10 min @ 2000 - 3000 rpm
  4. What specific component is serum lacking that plasma contains?
  5. What can cause hemolysis?
    • Moist syringe
    • Vigorous mixing
    • Forcing through needle
    • Freezing whole blood 
    • Too much pressure on the syringe 
  6. What causes lipemia?
    • Postprandial samples
    • Pancreatitis
    • Hypertryglyceridemia
    • Hypothyroidism 
  7. What does lipemia interfer with?
    Spectrophotometric measures
  8. What are 3 different analyzer types and which one is least affected by lipemia?
    • Colormetric
    • Spectrophotometer
    • Dry chemistry (least affected)
  9. Who requires maintenance logs for QC?
  10. When is a simple dilution performed?
    When a serum chemistry is too high to read on the machine
  11. What enzymes are released from damaged hepatocytes?
    • Alanine aminotransferase (ALT)
    • Aspartate aminotransferase (AST)
    • Sorbitol dehydrogenase (SDH)
    • Glutamate dehydrogenase (GLDH) 
  12. What enzymes are associated with cholestasis?
    • Alkaline phosphatase (ALP)
    • Gamma glutamyltranspeptidase (GGT) 
Card Set
Clinical Chemistries
Procedures, components of clin chem