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Compound Microscope
3 different lens systems
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Ocular Lens
- Closest to the eye
- Magnifies specimen 10x
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Pieces of the microscope moving top to botton
- ocular lense
- head
- revolving nose piece
- objective lenses
- stage
- condenser lens
- lever for iris diaphragm
- light source
- base
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Scanning Lens
- red
- 4x
- used to locate specimen
- has greatest field of view and working distance
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High dry lens
- blue
- 40x
- Highest power used with wet mounts
- total magnification is 400x
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100x oil immersion lens
- oil is necessary to prevent refraction of light
- Highest resolution
- total magnification 1000x
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Magnification
ability to increase the apparent size of something
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Resolution
Ability to distinguish objects which are close together, a combination of clarity and magnification
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Field of View
The diameter of the filed you see when you look through the ocular lens
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name the knobs used to focus a specimen
Coarse and fine adjustment knobs
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Working Distance
The distance between the tip of the lens and the specimen
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Depth of field
The vertical distance a specimen is in focus
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Stage Control Knobs
used to manipulate the slide along the stage
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Condenser Lens
Focuses light on the specimen
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lever for iris diaphragm
regulates the intensity of light passing through the specimen
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Parfocal
if a specimen is in focus under any power magnification then it should be approximately in focus with any power
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Parcentric
specimen will be approximately centered at all magnifications
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Trichinella spiralis
- worm that causes trichinosis, a muscular disease
- acquired by the consumption of raw or undercooked pork
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worm that causes trichinosis, a muscular disease acquired by the consumption of raw or undercooked pork
Trichinella spiralis
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saccharomyces cerevisiae
- yeast. common for bread, beer and wine
- circular budding cells
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yeast. common for bread, beer and wine circular budding cells
saccharomyces cerevisiae
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Mixed Diatoms
- microscopic algae
- responsible for 2/3's of earth's O2
- walls made of silicon "glass houses"
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Trypanosoma spiralis
flaginated single-cell organism is the cause of the African sleeping sicknesstransmitted by the bite of the tse-tse fly
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flaginated single-cell organism is the cause of the African sleeping sicknesstransmitted by the bite of the tse-tse fly
Trypanosoma spiralis
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black bread mold.This fungus has a large round ball at the end of a filament when the spores are ready the sporandium breaks ope to release them.
Rhizopus
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Rhizopus
- black bread mold.
- This fungus has a large round ball at the end of a filament
- when the spores are ready the sporandium breaks open to release them.
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Penicillium
- Mold- filamentous fungi
- some species provide penicillin
- others make cheeses
- The spore forming structures of this mold look like tiny paint brushes or fingers on a hand.
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filamentous Fungi - mold
some species provide penicillin
others make cheeses
The spore forming structures of this mold look like tiny paint brushes or fingers on a hand.
Penicilliun
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Aspergillus
- Mold- filamentous fungi
- serves as a pathogen
- produces pulmonary aspergillosis : respiratory infection
- Spore forming structure resembles a sun with rays radiating from it.
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Mold -filamentous fungi
serves as a pathogen
produces pulmonary aspergillosis : respiratory infection
Spore forming structure resembles a sun with rays radiating from it.
Aspergillus
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three basic shapes of bacteria
bacillus, cocci, spirilli
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Who and when were the Domains developed by. Name them.
- Woese, 1980
- Archaea
- Eubacteria
- Eukarya
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Name Whittaker's Kingdons, dev. 1970
- Monera
- Protista
- Fungi
- Plantae
- Animalia
- last three are grouped based on their means of obtaining nourishment
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Monera
- single-celled, prokaryotes
- no nucleus or complex cell structure
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Protista
- Unicellular and Multicellular
- animal-like unicellular organisms: trypanosoma
- plant-like: diatoms, algea
- fungus-like organisms: phytopthera
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Fungi
- yeasts and molds
- absorb nutrients from dead or living materials
- Pathogenic if absorbing from living
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Plantae
- Autotrophic (producing their own food) through photosynthesis
- Chlorophyll
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Animalia
- obtain nutrients through ingestion
- microscopic representation includes paracitic helmnths (worms)
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Why is the scanning lens used for locating a specimen?
It has the greatest field of view and working distance
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