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HIV tropism
- Macrophage tropic (CCR5)
- T Lymphocyte tropic (CXCR4)
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MO/EFFECTOR T-CELL TROPIC HIV
- CCR5 receptor binding (R5)
- Cell types with high CCR5 receptors:
- macrophage and effector T cells
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T-Resting/Effector T-cell tropic HIV
- CXCR4 receptor binding (X4)
- Cell types with high CXCR4 receptor:
- Resting cell T-lymphocyte and Effector T-cell
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attachment process
what HIV and host proteins are involved?
- CD4-gp120
- CXCR5/CCR5 helps to gp120 attachment
- gp41 harpoons cell membrane
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HIV genome activation in host cell signal
- NF-kappaB acivation acivates viral transcription
- same transcription factor activates the T-cell
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HIV assembly
see lidner slides
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HIV testing
- ELISA for anti-HIV-ab
- Western blot for same HIV for specific confirmation (anti-p24, gp41, gp120, gp160 HIV protein Ab)
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