IDC Mycoplasma/Legionella

  1. General Characteristics
    • Small pleomorphic
    • Slow growers
    • Highly fastidious
    • Need complex media
    • Mycoplasmas adhere to the epithelium of mucosal surfaces
    • Mycoplasma Class Mollicutes
  2. What are the 3 species of Human pathogens for Mycoplasma?
    • M. pneumoniae (Respiratory)
    • M. hominis (genital)
    • Ureaplasma (urogenital)
  3. How are the Human pathogens for Mycoplasmas transmitted?
    • Sexual contact
    • Mother to child
    • Respiratory secretions
    • Fomites
  4. How do you collect a specimen for Mycoplasmas?
    • No cell wall so are susceptible to drying
    • Immediate transport to lab is important
    • Swabs are placed with TSB 0.5% albumin and 400U penicillin
    • Specimen should be placed at -70C if not processed immediately
  5. What are the growth conditions for Mycoplasma?
    • Cultures are seldom ordered
    • Require trained personal (experts)
    • Only 40% sensitivity
    • Special media: SP4 broth/agar and 5-10% CO2
  6. What organism requires only SBA and arginine?
    M. hominis
  7. What organism requires SBA and urea and can also use Shepherd's 10 B Broth?
  8. What are more growth conditions required for Mycoplasma?
    • Blood Cultures need 1% gelatin to decrease toxicity of SPS
    • Collection must be ASAP (due to dryness)
    • Dracon and Ca alginate swabs with metal shaft
    • TSB with 0.5% albumin and 400U/mL of penicillin for transport
  9. How do you identify Mycoplasmas?
    • Like colonies stained with Dienes stain
    • Colonies have a typical fried egg appearance
    • Stained colonies have a light periphery and dark-blue center
  10. How long does M. hominis take to grow?
    2 days
  11. How long does M. pneumoniae take to grow?
    20 days
  12. What organisms grow in NY city agar as minute colonies, only observed with a stereoscope?
    • M. hominis
    • U. urealyticum
  13. What are the Serological procedures to identify Mycoplasmas?
    • Cold agglutinins (used for detecting atypical penumonia)
    • EIA and IHA detect both IgG and IgM
    • Complement fixation
    • Indirect immunofluorescence
    • PCR (very specific)
  14. Reference labs perform serology on what organisms?
    • M. hominis
    • U. ureolyticum
  15. Characteristics of Mycoplasma pneumoniae are:
    • Causes a typical pneumonia or "walking pneumonia"
    • 20% of the reported pneumonias
    • Higher incidence in young adults (not seasonal)
    • Groups at risk: college students, military
  16. Mycoplasma S/S?
    • Transmission is by aerosol droplets
    • Most infections asymptomatic or mild
    • Incubation period 2-3 weeks
    • Non-specific symptoms: sore throat, dry cough, fever, malaise, anorexia
    • Extrapulmonary infections are rare
  17. Characteristics of M. hominis and Ureaplasma urealyticum:
    • Associated with urogenital tract
    • Opportunistic pathogens
    • Higher rates in Africans (men and woman)
    • M. hominis found in 50% of healthy adults (interpretation difficult when asymptomatic)
    • Reported as cause of Non gonococcal urethritis
  18. What organism causes salpingitis, pelvic, inflamatory disease, and post-partum fever?
    M. hominis
  19. What organism causes 10% of cases of Non gonococcal urethritis in man and upper female genitourinary tract disorders?
    U. urealyticum
  20. What are the antimicrobials these organisms are susceptible?
    • Tetracycline
    • Erythromycin
Card Set
IDC Mycoplasma/Legionella