Microbio Midterm 1

  1. Early metabolism may have been catalyzed by _____
  2. The 1st person to visualize individual microbes was...
    Antoni Van Leeuwenhoek
  3. The use of agar as the gelling agent in solid media was suggested by whom?
    Angela Hesse
  4. The genetic expression machinery of archaea is most similar to _____
  5. Which of the folloing staining processes requires crystal violet?
    Gram Staining
  6. 2D polyacrylamide gel electrophoresis involves all of these except what? 

    C) DNA sequencing
  7. The chromosome includes a number of extra twists called supercoils. All of the following are true of supercoiling except what?

    B) In bacteria and eukaryotic cells, there is positive supercoiling
  8. Which name refers to having flagella attached at one or both ends of the cell?
  9. In the lab, E. coli can divide into 2 cells every _____
    20-30 mins
  10. Which is a micronutrient?

    C) zinc
  11. All of the following are true about MacConkey medium except what?

    E) Lactose fermenters appear colorless
  12. Which of the following can be used to assess protein expression under different culture conditions?

    A) 2D protein gels
  13. Okazaki fragments are approx. how many bases in length?
  14. Which molecule is responsible for removing torsional stress during DNA replication?
    DNA gyrase
  15. The eukaryotic predators of the microscopic world are ____
  16. Which of the following is not a palindrome?

    A) toto
  17. Which of the following is not rue about the 2 strands of DNA in a double helix?

    C) catenated
  18. Which of the following is not part of initiation of transcription?

    A) synthesis of a primer
  19. The energy souce utilized by the elongation factors EF-G and EF-Tu is ____
    GTP hydrolysis
  20. Post translational modification may involve

    D) all of the above
  21. Specific membrane components, particularly ________, determine which substances are transported across the membrane.
  22. Evolution toward pathogenicity may involve _____

    E) all of the above
  23. Hydrolysis of ATP with the release of pyrophosphate is the driving force of which of the following reactions?

    D) all of the above
  24. In glycolysis, dihydroxyacetone phosphate is converted to ____
  25. T or F: Antiseptics and antibiotics are both used orally to fight bacterial infections
  26. T or F: The bacterial nucleoid is bound by a membrane
  27. T or F: During biofilm formation, as more and more cells bind to the surface, they begin to send signals to each other in a process called quorum sensing 
  28. T or F: Extremophiles typically evolve to survive multiple extreme envirionments
  29. T or F: The ribosome has no enzymatic activity
  30. T or F: Only the nuclotide triphosphate ATP carries energy
Card Set
Microbio Midterm 1
Microbio Midterm 1 Questions