Lab quiz 6b.txt

  1. Enterics Gram
    gram negative rods
  2. Enterics oxygen requirement
    facultative anaerobe
  3. enteric fermentation
  4. enterics
    oxidase negative; catalase positive
  5. enterics spore?
    non-spore forming
  6. Salmonella disease
    enteric fever
  7. Shigella diseases
  8. Edwardsiella Tarda disease
  9. Yersinia enterocolitica disease
  10. yersina pestis disease
  11. enterobacter
    found in nature (soil, water...)
  12. Vibrio Cholerae disease
  13. Vibrio cholerae shape
    curved rods
  14. Urinary infections organisms
    Hafnia, Morganella, Procidencia
  15. Opportunistic enterics
    Erwinia and edwardsiella
  16. Enteric motility
    if motile- peritrichous flagellation
  17. Coliform
    G-, non sporeforming, facultative anaerobic rods which ferment lactose
  18. Non-coliform
    salmonella, shigella, proteus, and yersina which cannot ferment lactose
  19. E.Coli diseases
    UTI, montezuma's revenge, infant diarrhea
  20. first step to identify enteric
    select for their growth over other bacteria in the fecal sample
  21. Selenite F broth
    inhibits growth of microbial genera to select for enterics
  22. incubating to select for enterics
    incubated aerobically so obligate aerobes are inhibited
  23. selective media for enterics
    cotain bile salt, sodium deoxycholate, or dyes to inhibit G+ bacteria
  24. DCA and SS agar
    contain sodium citrate to inhibit the growth of coliforms (selective); contain lactose and natural red so lactose fermenters will prodice red colonies (differential)
  25. Differential Media
    allow us to differentiate between groups of bacteria
  26. Selective Media
    inhibit growth of unwanted organisms
  27. TSI
    differential media
  28. Yellow butt of TSI indicates
    glucose fermentation
  29. Yellow Slant of TSI indicates
    sucrose and/ or lactose fermentation
  30. Hydrogen Sulfide Production on TSI
    black slant
  31. Alkaline Reversion
    degradation of proteins when nutrients are exhaisted which neutralizes the acid and yields an alkaline appearance
  32. IMVIC test
    indole, methyl red, voges-proskauer, and inorganic citrate test
  33. Enterotube # of tests
  34. non-invasive
    dont invade tissues
  35. Opportunits
    cause disease if hosts defense is lowered or the balance is disturbed due to environmental factors
  36. pathogenic
    cause disease if they get past the hosts defense
  37. antimicrobials
    drugs that treat infectious diseases
  38. antibiotics
    one type of antimicrobial derived from living organisms
  39. three classifications of organisms due to size of inhibition
    resistant, intermediate, sensitive
  40. antibiogram
    pattern of resistance
  41. Bacteriocidal agents
    prevent growth and induce killing without lysis; bind tightly and aren't removed by dilution
  42. Bacteriolytic agent
    lyse cells
  43. Bacteriostatic agent
    inhibit growth; bind loose so with lowered concentration the growth may resume
  44. Synergism
    increased effect with two drugs given simulltaneously
  45. antagonism
    two drugs interfer with each others mechanism
  46. benefits of combining drugs
    provide optimal therapy and lessen the toxicity of individual drugs by reducing doasage of each
  47. benefits of kirby bauer
    efficeint and easy
  48. weakness of kirby bauer
    does not determine if a drug is bacteriostatic or bacteriocidal
  49. size of inhibition is affected by
    sensitivity of the organism, mw of organism, concentration of agent, density of culture, diffusion rate of agent, size of inoculum, time and temp, interactions b/w agent and medium
  50. Mueller-Hunton agar
    specific composition, support growth, buffer pH changes, not antagonistic, isotonic to blood, yield results, reproducible
  51. small antibiotics
    penicillin and ampicillin
  52. large antibiotics
    polymyxin and bacitracin
  53. small mlecules diffuse___
  54. large molecules diffuse___
  55. MIC
    lowest concentration that prevents visible growth
  56. MBC
    lowest concentration that prevents growth and induces killing
  57. negatives to broth dilution
    cumbersome and expensive
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Lab quiz 6b.txt
micro 221 lab quiz 6b