Lab Practical 2 - Sheet1.csv

Gram negative rods. Oxidase negative. Catalase positive. Facultative anaerobes. Ferment glucose. Usually cause GI diseases. Main difference is the ability to ferment Lacotose or Non lactose fermenting. Lac positive are usually normal flora and cause opportunistic infections nosocomial. Where as lac negative are normally outright pathogens.

Cannot ferment sorbital. Main difference between normal Escherichia coli. this can be distinguished by a SMAC plate. If the E.coli does not ferment the sorbital in the plate it is O156H7

Isolation of most Salmonella species. Selective agents. Brilliant green inhibits growth of Gram positives and most Gram negatives highly selective for Salmonella. DIfferential agents Lactose and sucrose bacteria that ferment these sugars produce yellowish colonies with yellow halo. Salmonella spp. do not ferment lac or suc colonies appear white with red halo

"Primary plating medium for Gram negative enterics. Selective agents Eosin & methylene blue dyes: select for growth of Gram negatives

"Primary plating medium for Gram negative enterics. Selective agents Bile salts inhibit growth of many Gram positives and non-enteric Gram negatives. Selective agents Sugars (lactose sucrose salicin): bacteria that ferment 1 or more of these sugars produce yellow/orange colonies

Primary plating medium for Gram negative enterics. Selective agents Bile salts and crystal violet inhibit growth of most Gram positives. Differential agents. Lactose: bacteria that ferment lactose produce bright pink colonies. E. coli (and some other enterics) precipitate bile salts and produce cloudy zones around the colonies

Isolation and identification of E. coli O157H7. Exactly same as MAC plate but has Sorbitol bacteria that ferment sorbitol produce bright pink colonies. Since O157H7 can not ferment this it will have buff colored colonies.

"Isolation and identification of Salmonella and Shigella species. Selective agents Biles salts and various dyes inhibit growth of most Gram positives. Differential agents Lactose bacteria that ferment lactose produce reddish colonies

"Glucose lactose and sucrose fermentation pattern Ferrous iron forms black precipitate if H2S is produced. Yellow butt red slant only glucose fermented

"Primary plating medium for Gram negative enterics especially useful for Shigella isolation/identification. Selective agent Deoxycholate (bile) inhibits most Gram positives. Selective agents Xylose bacteria that ferment xylose produce yellow coloniesH2S production bacteria that produce H2S form colonies with black centers. Shigella does not ferment xylose and appears red Salmonella ferments xylose and appears yellow

Used primarily for Lac negative Enterobacteriacae. Are the sugars Lysine. Orithine. Arnthenine. and a Control. Must use mineral oil on top to create anaerobic environment for fermentation. Works by removing carboxylic acids and can use them for an energy source making the solution basic. The tubes contain glucose plus one of the amino acids. If the bacteria can use the AA the solution will turn basic by breaking down the AA to an amine. The solution will turn back to its original blue color. yellow is a negative test.

Is an enzyme that removes an amine from phenylalanine. Uses teh enzyme deaminase to create phenyl pyruvic acid and an amine. To test this add ferric choloride to the slant and look for a BRIGHT GREEN COLOR.

TSI A/AG-. h2s negative. IDOLE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. vp negative. citrate negative. urease negative. maltose negative.

TSI A/AG-. h2s negative. IDOLE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. vp negative. citrate negative. urease positive. maltose negative. Main test SMAC PLATE WITH DULL COLORED COLINES. Not orange like normal e. coli.

A/AG -. ((H2S POSITIVE)). Indole negative. MOTILITY POSITVE. . MR POSITIVE. vp negative. ((CITRATE POSITIVE)). . UREASE POSITVE. ((MALONATE NEGATIVE)).

A/AG-. h2s negative. INDOLE POSITIVE. Motility positive. MR POSITIVE. . vp negative. CITRATE POSITIVE. UREASE POSITVE ((MALONATE POSITIVE). Between fruendi and diverusus use malonate and h2s and indole.

A/AG -. h2s negative. indole negative. MOTILITY POSITVE. mr negative. VP POSITVE. CITRATE POSITVE. urease negative. MALONATEPOSITIVE. VERY MOTILE!!!!

A/AG-. h2s negative. Indole negative. motility negative. mr negative. VP POSITIVE. UREASE POSITVE. CITRATE POSITVE. MALONATE POSITVE. to differentiate between citrobacter and klebisella use molilty and that citrobacter is MR positve. Remeber k. pneumonia is different from k. oxytoca by indole

A/AG-. h2s negative. ((INDOLE POSITIVE)). motility negative. mr negative. VP POSITIVE. UREASE POSITVE. CITRATE POSITVE. MALONATE POSITVE. to differentiate between citrobacter and klebisella use molilty and that citrobacter is MR positve. Remeber k. pneumonia is different from k. oxytoca by indole

Eserichia coli. Citrobacter deversus. Citrobacter freundii. Enterobacter areogenes. Klebisella pneumonia. Klebisella oxytoca.

SWARMER. K/AG+.(( H2S NEGATIVE)) SULFIDE POSITIVE. MOTILITY POSITVE. MR POSITIVE. vp negative. CITRATE POSITVE. UREASE POSITVE. PHEN. DEAMINASE POSITVE. Lysine decarb negative. arginine decarb negative. Ornithine decarb. Sucrose positve

SWARMER (((((A/A+))))). INDOLE POSITIVE. SULFIDE POSITIVE. MOLTILITY POSITIVE. MR POSITIVE. citrate negative. UREASE POSITIVE. . PHEN DEAMINASE POSITVE. Lysine decarb. Arginine decarb negative. Orithine negative. SUCROSE POSITIVE.

K/A-. INDOLE POSITIVE. (((SULFIDE NEGATIVE)))). MOTILITY POSITIVE. MR POSITIVE. vp negative. CITRATE POSITIVE. UREASE NEGATIVE. PHENYLDECARB POSITVE. lysine. ariginine ornithine negative. sucrose negative

PROTEUS IS A SWARMER AND HIGHLY MOTILE. ALL PHEN. DEAMINASE POSITIVE. MOTILITY POSITIVE. Proteus stuartii is the only H2S NEGATIVE. Proteus mirabolis is K/AG+ and indole and sucrose negative but citrate postive ornithine Positve. Proteus vulgaris is A/Ag+ and ferments sucrose. Is indole negative and cirtrate negative. ornithine negative.

K/AG-. indole negative. sulfide negative. motility negative. MR POSITVE. vp negative.urease negative. Phen. deaminase negative. lysine decarb. negative. ARGININE DECARB POSITVE. Orithine negative. Sucrose negative

K/AG-. indole negative. sulfide negative. motility negative. MR POSITVE. vp negative.urease negative. Phen. deaminase negative. lysine decarb. negative. arginine decarb negative.. ORINTHINE POSITVE. Sucrose negative

K/AG+. Indole negative. SUFLIDE POSITIVE. MOTILITY POSITIVE. MR POSITIVE. CITRATE POSITIVE. vp negative. Phenyl deaminase negative. LYSINE POSITVE ARGININE POSITIVE. ORNITHINE POSITVE. Sucrose negative

A/A- INDOLE POSITIVE. Sulfide negative. MOTILITY POSITIVE. MR POSITIVE. VP negative. Citrate negative. UREASE POSITIVE. Phen. deaminase negative. Lysine decarb negative. Arginine decarb negative. ORNITHINE POSITIVE. SUCROSE POSITIVE. IS NON MOTILE AT 35 DEGREE BUT MOTILE AT ROOM TEMP.

If it is PHEN. DEAMINASE POSITVE AND MOTILE (SWARMING) PROTEUS.. Negative everything except MR or One decarb AA is SHIGELLA. ALL POSITVE DECARBS IS SALMONELLA TYPHIMURIUM. If it is H2S negative and Indole positve and phen. negative but sucrose postive is Yersina enterocolitica.

Look for H2S production. Non H2S is Y. stuartii. Only H2S positive with A/AG+ is P. vulgaris and this is because of SUCROSE. P. mirabilis is positive for citrate and negative indole and positive ornithine.

Gram negative s shaped. can be helical. OXIDASE POSITIVE. Microaerohilic. When staining it is hard to do secondary stain and must use carbofusion instead of safranin.

Isolation and growth of Campylobacter especially C. jejuni. Selective agents Antibiotics (cefoperazone vancomycin amphotericin B) inhibit growth of other bacteria. Contains sheep blood

Isolation and growth of Campylocbacter species. Antibiotics (cefoperazone vancomycin cyclohexamide) inhibit growth of other bacteria. Blood-free medium charcoal acts as a quencher of reactive O2 species

Isolation and enumeration of marine bacteria. selective High salt concentration: selects for halophilic bacteria. No differential agent Vibrio cholerae is not halophilic and will not grow! All other Vibrio species should grow

"Isolation and identification of Aeromonas species. Maltose colonies that ferment maltose appear yellow

"Primary isolation of Vibrio species particularly V. cholerae. Bile salts inhibit Gram positive organisms

NALIDIXIC ACID SENSITIVE. (disk placed on campy CVA agar) cephalothin resistant (disk on campy CVA). GROWTH AT 42 POSITIVE. HIPPURATE POSITIVE.

Nalidixic acid negative. CEPHALOTHIN SENSITIVE. Growth at 42 negative. hippurate negative