-
Phenylethyl alcohol agar
- inhibitor - phenylethyl alcohol
- inhibits gram (-) growth by breaking down membrane
- selective
-
Columbia CNA w/5% sheep blood
- inhibitor - colistin and nalidixic acid
- inhibits gram (-) by affecting membrane integrity and interfering with DNA replication
- indicator - blood
- both
- positve - good growth with clearing or greening
-
Bile esculin agar
- inhibitor - bile salts
- separates the streptococcu group and enterococci from othe streptococci
- indicator - ferric citrate
- both
- positive - darkened media
-
Mannitol salt agar
- inhibitor - sodium chloride
- dehydrates and kills most bacteria
- indicator - phenol red
- both
- positive - bright yellow colonies with yellow halo
- can also tell the pH of the organism
-
MacConkey agar
- inhibitors - bile salts & crystal violet
- inhibits gram (+)
- indicator - phenol red
- both
-
Eosin Methylene Blue agar
- inhibitor - lactose & sucrose
- inhibit gram (+)
- indicator - neutral red dye
- both
- positive - pink or dark growth
-
Hecktoen enteric agar
- inhibitor - bile salts
- inhibit gram (+) and enterics
- indicator - ferric ammonium citrate, bromothymol blue, acid fuchsin
- both
- sulfer reduction is indicated by blue/green growth with black precipitate
-
Xylose lysine desoxycholate agar
- inhibitor - desoxycholate
- inhibits gram (+)
- indicator - phenol red
- both
-
oxidation-fermentation test
- differentiate bacteria based on their ability to oxidize or ferment sugars
- stab inoculated then sealed with oil
- 2 tubes
- pos - yellow
- neg - green or blue
-
phenol red broth
- fermentation with acid and/or gas
- 4 tubes
- broth inoculated
- pos - yellow
- neg - red
-
methyl red and VP test
- methyl red - detect if organisms capable of performing a mixed acid fermentation
- VP - organisms are capable of fermenting glucose
- 3 tubes
- broth inoculate
- add reagents
- pos - red
- neg - no color change
-
catalase test
- identify organisms that produce catalse
- one
- slant streak
- add hydrogen peroxide
- pos - bubbles
- neg - no bubble
-
oxidase test
- identify bacteria containing oxidase
- wooden end of sterile cotton applicator
- reagent slide
- pos - dark blue
- neg - no color change
-
nitrate reduction test
- reduction of nitrate to nitrite
- 1 tube
- broth inoculate
- add 8 drops of reagent A and B and zinc dust
- pos - gas/ red after reagents
- neg - red color after zinc
-
citrate test
- determine the ability of an organism to use citrate as its sole source of carbon
- 1 tube
- light inoculum streak with needle
- pos - blue or no color change with growth
- neg - no color change or growth
-
Malonate test
- ferment or utilize malonate
- 1 tube
- broth inoculate
- pos - dark blue
- neg - no color change
-
decarboxylase test
- if the organism produces decarboxylase enzyme
- 4 tubes
- streak
- add mineral oil
- pos - purple color
- neg - no color/yellow
-
Phenylalanine deaminase test
- if the organism contains phenylalanine deaminase
- 1 tube
- streak with heavy inoculum
- add drops of ferric chloride after incubation
- pos - green
- neg - no color change
-
Starch hydrolysis test
- if amalyse is or isnt present
- one plate for all
- spot-inoculated
- add iodine after
- pos - clearing around growth
- neg - no clearing
-
Urea hydrolysis
- differentiates rapid urease (+) bacteria from slower urease (+) and urease (-) bacteria
- one tube and one plate
- heavy broth inoculate and streak inoculate
- read 24 and 48 hours - re incubate (-)
- pos - pink
- neg - orange/yellow
-
Gelatin hydrolysis test
- ability to produce gelatinases
- one tube
- heavy stab-inoculate
- reicubate if liquid
- pos- liquid
- neg - solid
-
DNA hydrolysis test
- ability to produce DNase
- one plate for all
- spot-inoculate
- pos - clearing around growth
- neg - no clearing
-
SIM medium
- identify bacteria capable of producing indole, usign the enzyme tryptophanase
- combination - sulfer reduction and motility
- one tube
- stab-inoculate with needle
- add kovacs' reagent after incubation
- pos - red in alcohol layer of reagent, black in medium, growth radiating out
- neg - reagent color unchanged, no black, no radiating growth
-
Triple sugar iron agar
- differntiate bacteria ont he basis of glucose fermentaiton, lactose fermentation, sucrose fermentation, and sulfur reduction
- combination - animal proteins as sources of carbon and nitrogen, both ferrous sulfate and sodium thiosulfate as sources of oxidized sulfur
- one slant
- heavy streak, stab the butt
- pos - yellow or red, black
- neg - no color, no precipitate
-
Lysine iron agar
- differentiate enterics based on their ability to decarboxylate or deaminate lysine and produce hydrogen sulfide
- combination - triple sugar iron agar
- one slant
- stab the agar and streak the slant
- pos - purple/ red, black precipitate
- neg - yellow
-
Blood agar
- isolationa nd cultivation of many types of fastidious bacteria
- one plate
- quadrant streak
- pos - clearing or greening
- neg - no change
-
coagulase test
- presence of either bound or free coagulase enzymes
- one tube
- broth inocualtion
- mix tubes
- pos - clumping, solid media
- neg - no clumping, liquid
-
motility test
- motility
- one tube
- stabe inocualte
- pos - radiatiing growth
- neg - no radiating growth
-
thiogllycoate medium
- promote growht of a wide variety of fastidious microorganisms
- one tube
- broth inoculate with gentle mixing
- pos results only
- -obligate aerobic - top only
- -obligate anaerobic - bottom only
- -facultative bacteria - all throughout but more at top
- -microaerophile - top and a little below
- aerotolerant - all evenly through out
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