Bio Presentations

  1. K. De Wael, L. Lepot, F. Gason, B. Gilbert
    • --In Search of Blood - Detection of Minute Particles of Blood Using Spectroscopic Methods FSI Vol. 180, No. 1, 2008.
    • --Brian and Dustin
    • --Raman spectroscopy used typical spectrum of hemoglobin to identify blood particles
  2. Stacey Anderson, Brandi Howard, Gerald R. Hobbs, Clifton P. Bishop
    • --A Method for Determining the Age of a Bloodstain (using RNA) FSI Vol. 148, No. 1, 2005.
    • --Jen and Anthony
    • --identify age of bloodstain
    • --used real-time reverse transcriptase PCR to show that the ratio between different types of RNA (mRNA versus rRNA) changes over time in a linear fashion when dried human blood from eight individuals was examined over the course of 150 days
  3. Hiroaki Nakanishia, Akira Kido, Takeshi Ohmori, Aya Takada, Masaaki Hara, Noboru Adachi, Kazuyuki Saito
    • --Identification of Saliva by Detecting Oral Streptococci FSI Vol. 183, No. 1, 2009
    • --Joli and Daniela
    • --used DNA amp. to identifiy common strains of bacteria and then identify the presence of saliva
    • --data indicated that S. salivarius is more reliable than S. mutans
  4. Robert Driscoll, Dane T. Plaza, Donia Slack, and Robert A. Bever
    • --The Screening of Buccal Swabs (and/or saliva stains) with Ninhydrin Resulting in Improved Cell Collection AAFS Proceedings, A93, 2009.
    • --Tara and Jess
    • --used ninhydrin to detect amino acids found in saliva on buccal swabs
  5. Barni F, Berti A, Rapone C, Lago G.
    • --Alpha-Amylase Kinetic Test in Bodily Single and Mixed Stains JFS Vol. 51, No. 6
    • --Erik and Sharon
    • --used a quantitative spectrophotometric technique to determine alpha-amylase activity and reaction kinetic curves
    • -- recognize successfully the presence of saliva and to distinguish it specifically from other bodily fluids containing alpha-amylase
    • --clear correlation between alpha-amylases activity and male DNA was detected on all the samples evaluated
  6. Lowenstein JM, Reuther JD, Hood DG, Scheuenstuhl G, Gerlach SC, Ubelaker DH
    • --Identification of Animal Species by Protein Radioimmunoassay FSI Vol. 159, No. 2, 2006
    • --Breahna and Shannon
    • --pRIA was used to identify the species of six bone fragments lacking morphological specificity and 43 bloodstained lithic tools
    • --conclude that the pRIA technique shows a high degree of accuracy in discriminating human from animal bone fragments and bloodstains and in identifying animal species
  7. Kelly Virkler, Igor K. Lednev
    • --Raman Spectroscopic Signature of Semen and its Potential Application to Forensic Body Fluid Identification FSI Vol. 193, 2009
    • --Bethany and Nancy
    • --used Raman spectroscopy to create signature semen spectra and identify unique components in semen
  8. Jessica V. Norris, M.Sc.; Kate Manning, Bsc.; Sarah J. Linke, B.S.; Jerome P. Ferrance, Ph.D.; and James P. Landers
    • --Expedited, Chemically Enhanced Sperm Cell Recovery from Cotton Swabs for Rape Kit Analysis JFS Vol. 152, No. 4, July 2007
    • --Ciara and Christine
    • --exploits the exclusive use of detergents for intact cell removal, and can be utilized in conjunction with, or to circumvent, conventional differential extraction
    • --Samples treated with Sarkosyl and sodium dodecyl sulfate yielded higher sperm cell recoveries
    • --results indicate that the exclusive use of SDS enhances the release of sperm and epithelial cells from a cotton swab as compared with DE buffer, providing for a more effective DNA analysis
  9. Kazunori Sagawa, Akihiko Kimura, Yoshifumi Saito, Hiroshi Inoue, Seiji Yasuda, Mizuho Nosaka and Tsutomu Tsuji
    • --Production of a Monoclonal Antibody for Sweat-Specific Protein and its Application for Sweat Identification Int J Legal Med Vol. 117, No. 2, 2003
    • --Gretchen and Elyse
    • --identified a sweat-specific protein and produced monoclonal antibodies by immunizing mice with sweat proteins fractionated by anion-exchange chromatography
    • --one monoclonal antibody (G-81) was selected because of its unique specificity
    • --The G-81-reactive peptide was very stable and was able to detect sweat stains left for at least 11 weeks at room temperature without substantial loss of reactivity
  10. Koichi Sakurada, Tomoko Akutsu, Hisayo Fukushima, Ken Watanabe, Mineo Yoshino
    • --Detection of Dermcidin for Sweat Detection by RT-PCR and ELISA FSI Vol. 194, 2010
    • --Candace
    • --demonstration of the use of real-time RT-PCR to sensitively identify sweat among body-fluid stains, and it confirmed that dermcidin was an excellent marker for sweat identification
  11. Bauer M, Patzelt D.
    • --Identification of Menstrual Blood by Real Time PCR FSI Vol. 174, No. 1, 2008
    • --Amanda and Melissa
    • --method using real time-PCR and the menstrual blood marker MMP-7 which increases sensitivity and specificity
  12. Rachel I. Fleming, SallyAnn Harbison
    • --The Use of Bacteria for the Identification of Vaginal Secretions FSI: Genetics, In Press, 2010
    • --Nick and Abby
    • -- used the 16S–23S rRNA intergenic spacer region for identifying vaginal specific bacteria
    • --demonstrated that these Lactobacilli are promising new markers for the forensic identification of vaginal secretions
  13. Sarah K. Paterson, M.Sc.; Cynthia G. Jensen, Ph.D.; Susan K. Vintiner, B.Sc.(Hons); and Susan R. McGlashan, Ph.D.
    • --Immunohistochemical Staining as a Method for the Identification of Vaginal Epithelial Cells JFS Vol. 51, 2006
    • --Kristyn and Hyung Won
    • -- to use a cell extraction procedure compatible with routine forensic sampling methods, and to investigate the expression of cytokeratin (CK), estrogen receptor-α (ERα), and phosphodiesterase 5 (PDE5) in order to distinguish between skin, buccal, vaginal, and external penile epithelial cells
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Bio Presentations
Forensic Biology Class Presentations