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name liver/ glall bladder assays
- ALT
- ALP
- AST
- ammonia
- bile acid
- bilirubin
- BSP
- Cholesterol
- GGT
- SDH
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name the protein assays
- TP
- Albumin
- Globulin
- A/G ratio
- Fibrinogen
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we test ___ by putting into a EDTA tube, centrifuging, and drawing off supernatant.
Plasma
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We tests ___ by allowing it to clot for 30 minutes, centrifuging, and drawing off supernatent.
serum
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things that alter blood chemistry
- lipemic
- hemolysis
- ictherus
- medications
- hyperproteinemia
- dehydration
-
how do we test for TP
refractometer and chem profile
-
tested by bromcresol green dye binding for chem profile
albumin
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tested by measuring the diff in TP and albumin
globulin
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heat precipitation test for chem profile on __ using an EDTA tube
fibrinogen
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screener test for liver dz. Its numbers can level out in a few days, normally.
ALT
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non specific liver damage that can mean liver dz or necrosis. Its numbers will gradually change.
AST
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liver enzyme that we look for in large animals for hepatocellular damage or necrosis
SDH
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a liver enzymes found more in younger animals. can be found from cartilage, placenta, and bones
ALP
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a liver specific enzyme (period)
GGT
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bilirubin is _a_?
it is found in 2 forms. What conditions are associated with each?
breakdown product of hemolysis
- Conjugated: bile obstruction
- Unconjugated: jaundice, liver failure, open bile duct
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enzymatic test that will control fat break down and stabilization of cholesterol levels.
bile acids tests
-
bile duct obstruction is called what?
what conditions are associated with this?
cholestasis
DM, cushings dz, hypothyroid
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a liver test in which you can not use whole blood because the RBC will release nitrogen which will artificially raise the levels.
what tube do we use instead?
ammonia
free heparinized tube
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name kidney assays
- BUN
- Creatinine
- BUN/ Creatinine
- Urine Protein/ Creatinine
- Uric Acid
- GFR tests
-
BUN stands for ...?
blood urea nitrogen
-
which three bacterial infections can cause an increase in urease and hence increase BUN
- 1- Staff. aureus
- 2- Proteus
- 3- Klebsiella
-
a by-product of muscle breakdown that lets us know how the glomerulus is working
creatinine
-
a ratio that reflects GFR that should be less that 1.
BUN/ Creatinine ratio
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ratio in which cystocentesis is necessary.
Protein/ Creatinine ratio
-
a kidney assay more so for avians thats shows their typical breakdown of nitrogen
uric acid
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name pancreatic assays (9)
- amylase
- lipase
- trypsin
- insulin
- glucose
- fructosamine
- insulin tolerance
- glucose tolerance test
- glucagon
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name exocrine pancreatic assays
- amylase
- lipase
- trypsin
- insulin
-
found in pancrease, salivary glands, and small intestine that breaks down starches and glycogen.
what conditions cause an increase in its levels?
what tube do we never use?
amylase
pancreatitis, enteritis, ulcers of small intestine
dont use calcium binding ABT tube
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filtered pancreatic enzyme that is filtered through the kidney that increases in pancreatitis and pancreatic dz
lipase
-
enzyme that breaks down protein in food that we look at to identify pancreatic insufficiency
trypsin
-
enzyme we monitor to identify insulinoma
insulin
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test we use to find the wave of glucose levels
-
name adrenal gland assays (3)
- ACTH stimulation test
- Dex Suppression test
- CRH
-
-
an adrenal gland tumor can cause ___
cushings or addisons dz
-
name pituitary assays (2)
- GH evaluation
- GHRH response
-
increases GH will cause
agromegaly: increased growth
-
name 7 important electrolyte tests
-
Using a Ca containing tube to get Ca levels will ...
artifically increase Ca
-
Electrolyte found in bone, some RBC and has an inverse relationship with Ca.
P
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hemolysed rbcs with ___ P number
increase
-
electrolyte that pulls water and is usually accompanied by Cl
which tube do you never use to test this electrolyte?
Na
heparinized salts (green tube)
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electrolyte in which we use plasma to test because platelets will release it
K
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electrolyte in every tissue
Mg
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electrolyte that accompanies Na
hemolyzed rbcs will ___ this electrolyte
Cl
increase
-
a electrolyte that is measured by CO2.
It is a buffer and will ___ if you let it sit
where is the best place to get a sample
bicarb
change due to glycolysis
artery
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name misc assays (7)
- CK
- Lactate
- Gastrin
- Fecal
- Lipid absorbtion
- Folate
- Cobalamin
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a muscle wasting found in hourse and cattle down on ground causing damage. It usually can not be localized.
what condition can usually cause this?
CK or creatinine kinase
myocytis
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anaerobic metabolism may mean not enough oxygen. Bacterial activity that breaks down RBCs.
lactate
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stomach activity test for ulcers and GI function and lipid metabolism
gastrin
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jejunum absorption
folate
-
ileum absorbsion and measurement
cobalamin
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dark stool bc of early bleeding in stool that became digested
melena
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frank blood seen in or on stool
hematochezia
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toxicology assays (5)
- lead
- nitrate/nitrite
- rodenticides
- hb denaturation
- ethylene glycol
-
basophilic stippling on RBCs means
lead poisoning
-
pigs, horses, and cattle eat stuff with ... in feed and get poisoning
nitrate/ nitrite
-
warfaring disruption of vitamin K needed for clotting factors 2, 7, 9, 10
rodenticides
-
CO, onions in dogs, maple leaves in horse, new methylene blue in cats cause
Hb denaturing
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ethylene glycol is commonly known as ___
it causes what condition
antifreeze
irreversible kidney dz
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