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Ioninzing forms of radiation such x-rays, cause mutations by
creating ions pairs that lead to abnormal chemical reactions
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A collection of clones that contains at least one copy of every DNA molecules that was synthesized from mRNA is called a (an)
Complementary DNA library
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The reason that a vector must carry a gene that provides antibiotic resistance to its host is so that it can
allow the selection of the host cells that have taken up the vector and its rcDNA.
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The reason that a vector is engineered to contain unique restriction sites is to
allow the vector and the DNA that will be inserted to be cut in an identical manner.
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The reasonb that a vector must contain the Ori gene is to
allow the DNA that is inserted to be replicated
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an enzyme that recognizes a specific base sequence in DNA and makes a nonspecific cut justt outside of that sequence
restriction DNA endonucleases type I
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If a protein is going to be secreted from the cell after translation, which of the following is likely to be a compenent of its newly synthesized polypeptide chain?
answers?
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(in the previous answer) the molecule ___ binds to a molecule called the___
Answe?
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Name the vector best suited to carry a 40kb segment ot rcDNA
a cosmid
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a filtration column that contains beads that have poly-T sections attached is best used for
collecting eukaryotic mRNA molecules
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Which of the following vectors inserts recombinant DNA into host cells by transduction
Phage Lambda
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Which of the following vectors insert recombinant DNA into host cells by transformation?
pUC18
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If we wanted to synthesizes DNA and mRNA that we collected, we would FIRST add
poly-T primer
-
If we wanted to synthesizes DNA and mRNA that we collected, we would SECOND add
RNA-dep. DNA polymerase
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The pUC-19 vector was engineered so that the vectors that do not incorporate the rcDNA insert will still have a functional lac Z+ gene that will produce blue colonies in E.coli transformats; however, vectors that do incorporate the rcDNA insert will not have a functional lac Z+ gene, producing transformant that will
Allows the selection of the host cells that have taken up rcDNA plasmids.
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The photoactivated repair system works by
-
The excision repair system works by
Using DNA endonuclease, polymerase and ligase activity to cut out the thymine-Thymine dimers and replace the damaged DNA.
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They type of mutation that results in uncommon base pairing caused by hydrogen atom moving to an uncommon (rare state) position in the same nitrogen base, is call a (an)
Tautomeric Shift
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The loss of a nucleotide from a gene may result in change in th e rading of its base sequence , for example CATCATCAT_ATCATCACTACT. This type of mutation is called a (an)
frame shift
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Non-ionizing forms of radiation such as ultraviolet light, causes mutations by
energizing the electrons of bases such that they form abnormal chemical bonds.
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