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a sensitive test for deteection of antibody
Agglutination
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precipitation test are sensitive in detecting ?
antigen
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precipitation
antibody reaction with a soluble antigen
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Agglutination
antibody reaction with insoluble (particulate) antigen
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lysis
binding of antibody to lysable cell (bacteria or erythrocytes) and the subsequet acton of complelmement.
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What are the precipitation reactions?
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What are the kinds of precipitation tests?
- C-reactive protein precipitation test
- Double diffusion precipitation test in agar gel (ouchterlony method)
- immunoelectrophoresis
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immunoelectrophoresis
a technique combinging intial electrophorectic separation of proteins followed by immunodiffusion with resultant precipitation arcs
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C-reactive protein (CRP)
a sensitive marker of systemic inflammation
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What is a powerful predictor of cardiovascular disease?
CRP (C - reactive protein)
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What is one approach to the detection of C-reactive protein in the serum?
capillary tube precipitation test
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CRP is an (antigen or antibody)?
antigen
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Anti-CRP is an (antigen or antibody)?
Antibody
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In the double diffusion precipitation test in Agar gel, what is in well #1?
rabbit antiserum to human serum
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In the double diffusion test in agar gel, what is in well # 2?
undiluted, pooled, normal human serum
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In the double diffusion test in agar gel, what is in well # 3?
human gamma globulin solution (Cohn's Fraction II)
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in the double diffusuion tests, what is the rabbit antiserum to human serum? (antigen or antibody?)
antibody
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in the double diffusuion tests, what is the undiluted, pooled, normal human serum? (antigen or antibody)
antigen
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in the double diffusuion tests, what is the human gamma globulin solution (Cohn's fraction II) ? (antigen or antibody)?
antigen (IgG)
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