DNA Genomics 1 (MJC)

DNA replication occurs through the

1) Conservative Hypothesis
2) Semi-conservative Hypothesis
3) Dispersive Hypothesis

The main function of helicase is to

1) prevent reannealing of the DNA until new complementary DNA strands are synthesized
2) provide the free 3' OH group for DNA polymerase III to initiate DNA synthesis
3) proofreads the previous region to ensure proper base pairings have occurred
4) unwind DNA by breaking the hydrogen bonds between the complementary bases

In eukaryotic cells, the TATA box is found within the

1) Promoter region
2) Transcription unit
3) Terminator region
4) None of the above

Enzymes are needed during DNA replication.
Examples of these enzymes are

1) RNA polymerase, DNA polymerase I and helicase
2) aminoacyl-tRNA synthetase, helicase and DNA polymerase III
3) Primase, helicase and DNA ligase

During DNA replication, replication on the leading strand is _________ while on the lagging strand, it is __________.

1) continuous, continuous
2) discontinuous, discontinuous
3) continuous, discontinuous
4) discontinuous, continuous

One mechanism of DNA repair is known as excision repair.
This involves

1) hydrolysing the daughter strand containing the wrong nucleotides and synthesizing a new strand
2) cutting out the damaged segement and replacing it with the properly paired nucleotides
3) hydrolysing both the parent and daughter strand and synthesizing a new DNA double helix

The promoter region is the region of DNA ___________________________________________.

1) where RNA polymerase binds to to initiate transcription
2) that is transcribed into an RNA molecule
3) thats signals the end of transcription

All codons code for a specific amino acid except for

1) UCA
2) UAC
3) UGA
4) UAG

In order for an amino acid to be used for the translatin of a protein, it must first be activated.
This activated state is called

1) aminoacyl-tRNA complex
2) aminoacyl-tRNA synthetase
3) aminoacyl-AMP-enzyme complex
4) aminoacyl-tRNA binding site

A ribosome have 3 sites which are needed for the translation of proteins.
An example of the sites are

1) Peptidyl-tRNA binding site
2) Aminoacyl-tRNA binding site
3) Exit site
4) All of the above

The start codon codes for the amino acid methionine. An initiator tRNA with the anticodon, ___, will base pair with the start codon on the mRNA.

1) AUG
2) UAC
3) UCA
4) AGU

During transcription of the DNA fragment shown, a single base is paired incorrectly.
-C-T-A-A-C-G (sense strand)
-G-A-T-T-G-C
Which mRNA strand results?

1) C-T-A-A-G-G
2) G-A-U-U-C-C
3) G-A-U-U-G-C
4) G-T-U-U-C-C

What is an advantage of DNA having complementary strands?

1) Diploid cells can inherit DNA from both parents.
2) Semi-conservative replication is possible.
3) Replication and transcription can occur at the same time.
4) Synapsis of homologous chromosomes can occur easily.

If there are 243 amino acids and DNA only contains three types of nitrogenous bases, what is the minimum number of bases per codon that can code for each amino acid?

1) 3
2) 4
3) 5
4) 6

Insulin is made up of 2 polypeptide chains, one consisting of 20 amino acids, the other 31.
What is the minimum number of base pairs of DNA required to code for this molecule?

1) 20
2) 51
3) 62
4) 153