which one is more abundant codon or anticodon
codon. some tRNA (has anticodon) can recognize more than one codon (wobble hypothesis).
which site of anticodon, codon is not very specific
The 3rd position of the codon ( or the 1st base of the anticodon)
I on the 1st base of the anticodon can bind to
C, A, U of the 3rd position codon
where in the tRNA does aa attach? what kind of bond?
to the 3' ( or2') end of the anticodon tRNA. OH is on these ends. Ester bond is formed from interaction of the OH (tRNA) and the carboxyl group (aa)
enzyme use in ester bond formation of the aa and tRNA
what kind of bond? what is used up here?
aminoacyl-tRNA synthetase, covalent bond, ATP-->AMP
what drives the ester bond formation of tRNA and the aa? what enzyme used to form this?
pyrophosphate is degraded to inorganic phosphate by pyrophosphatase
how many different kinds of aminoacyl-tRNA synthetases are there?
20. (specific for each aa)
the first aa made in the prokaryote protein
direction of ribosome movement
5'-3' of mRNA
(DNA and mRNA are synthesized in 5'-3' and protein is synthesized in 3'-5' direction)
which ribosome is longest ? bound to 5' end mRNA or 3' end of mRNA
ribosome bound to 3'mRNA is longest and most complete
These two things are required for translocation
EF-G and GTP(hydrolysis)
Elongation factor corresponding to EF-G in eukaryotes
high concentration of this removes the ADP-ribose from EF-2 (this is the reverse rxn of ADP ribosylation)
Diphtheria toxin is produced by
Corynebacterium diphtheriae ( the toxin transfers ADP ribose to EF-2)
mRNA translation mutation