micro lab 1 practical 1

(15x) mag

• red scanner (4)
• yellow scanner (10)
• blue high dry (40)
• white oil immersion (100)

A condenser is a lens that serves to concentrate light from the illumination source

1) lower light source 2)lower stage all the way 3) scanner obj in place 4)wrap cord neatly 50dust cover

between distance of eyes and lens

lens stay focused when lens changed

ability to measure spepartion of images that are close together

speed of light as it goes thru an object

archaea and bacteria(do not have any nucleus,mitrochondria,or any membrane bound organelles.

protists & fungi (microscopic) plantae anamalia (have nucleus contain complete structures enclosed in nucleus)

everywhere present

one oraganism lives off another

one organism lives on another and is unharmed

ability of a non pathogenic 2 act as pathogenic in certain circumstances

a liquid medium containing a variety of nutrients 2 grow cultures of microoraganisms

introduce microorganisms 2 culture medium

controlled enviroment temp on medium so they multiply

cloudy

(BAP) special nutrients

(TSA) triple soy agar

(SDA) allows some 2 grow but not all

able to differentiate b/w bacteria based on the reaction on the plate

triple soy agar

blood agar plate

sabouraud dextose agar

partial breakdown

complete rupture

NO CHANGE

free of pathogens

free of all organic organisms

the process of killing pathogenic organisms

is to ensure that no microorganisms enter the cultures

It is used to sanitize medical and other biohazardous waste before it is discarded with non-hazardous waste

we want to isolate to get a pure colony

eukarotic-unicellular, reproduce by budding

branches

sections of the branching(mycelium)

on hyphale(sections of branching) molds reproduce by spores

yeasts reproduce by budding

from yeast to mold with temp change

are fungi that grow in the form of multicellular filaments called hyphae become mycelium

The fungal cell wall is composed chitin and r heterotrops enzymes digest then ingest nutrients

has a neg charge.bacterial repels neg charge dont take up dye and shows shape since background is dyes

• 130secs crisle violet-stains gram/neg and gram pos.peptidoglycan layers purple.
• 21min(mortant)grams iodine insoluble complex with cristle complex
• 310secgrams alchol removes crisle violet from gram neg bacteria making peptogycan colorless shrinks pores of peptidoglycan layer in gran pos bacteria purple
• 41minsaferine is applied and stains decolorized pink gram pos unchanged and still purple