ability of a non pathogenic 2 act as pathogenic in certain circumstances
broth
a liquid medium containing a variety of nutrients 2 grow cultures of microoraganisms
innoculate
introduce microorganisms 2 culture medium
inculbate
controlled enviroment temp on medium so they multiply
turbulancy
cloudy
enriched
(BAP) special nutrients
all-purpose
(TSA) triple soy agar
selective
(SDA) allows some 2 grow but not all
differential
able to differentiate b/w bacteria based on the reaction on the plate
tsa
triple soy agar
bap
blood agar plate
sda
sabouraud dextose agar
alpha
partial breakdown
beta
complete rupture
GAMMA
NO CHANGE
asptic
free of pathogens
sterilization
free of all organic organisms
disinfection
the process of killing pathogenic organisms
aspectic techniques
is to ensure that no microorganisms enter the cultures
incinerators purpose
It is used to sanitize medical and other biohazardous waste before it is discarded with non-hazardous waste
how the streak plate is used to onbtain a pure colony
we want to isolate to get a pure colony
yeast charteristics
eukarotic-unicellular, reproduce by budding
define mycelium
branches
define hyphale
sections of the branching(mycelium)
define spores
on hyphale(sections of branching) molds reproduce by spores
define budding
yeasts reproduce by budding
define dimorphic
from yeast to mold with temp change
def mold
are fungi that grow in the form of multicellular filaments called hyphae become mycelium
charteristic of fungi
The fungal cell wall is composed chitin and r heterotrops enzymes digest then ingest nutrients
def neg stain
has a neg charge.bacterial repels neg charge dont take up dye and shows shape since background is dyes
gram stain process
130secs crisle violet-stains gram/neg and gram pos.peptidoglycan layers purple.
21min(mortant)grams iodine insoluble complex with cristle complex
310secgrams alchol removes crisle violet from gram neg bacteria making peptogycan colorless shrinks pores of peptidoglycan layer in gran pos bacteria purple
41minsaferine is applied and stains decolorized pink gram pos unchanged and still purple