Clinical Chemistry

  1. photons of energy that travels in waves
    Electromagnetic radiant energy
  2. beam of electromagnetic radiant energy
    linear distance traveled by one complete wave cycle
    Wavelength (λ)
  3. speed of a wave cycle
  4. # of wave cycles occurring/second
    Formula: v = c/ λ
  5. atoms, ions or molecules have ability to absorb photons
    of light
  6. λ at which molecule will absorb maximum amt. of energy
    excitation λ
  7. as “excited” ions, atoms or molecules return to ground state
    Emission process
  8. What are the three forms of relesed energy?
    kinetic, vibrational, radiant light (flourescence)
  9. ratio of transmitted radiant energy (Is)/radiant incident light on sample (I)
  10. contains everything but solute being measured
    sets a new intensity of light Io
    reference solution
  11. What is the amt. light absorbed dependent on?
    • molecular structure
    • reaction between molecules & radiant energy
  12. The concentration of a substance is directly proportional to the amount of light absorbed or inversely proportional to the logarithm of the transmitted light.
    Beer's Law
  13. The ratio of radiant energy transmitted divided by the radiant energy incident on the sample.
    %Transmittance (%T)
  14. What are the lamps most commonly used for the spectrophotometer? (visible and near-infrared)
    • Incandescant Tungsten
    • tungsten-iodide
  15. What are the lamps most commonly used for ultraviolet work on the spectrophotometer?
    • Detrium-discharge lamp
    • Mercury-arc lamp
  16. Name the part by its function:
    Isolation of individual wavelengths of light.
  17. What are some examples of monochromators?
    • Colored glass filters - most commonly used
    • Interference filters
    • Prism
    • Diffraction
  18. Convert the transmittance radiant energy into an equivalent amount of electrical energy.
  19. What are some examples of photodetecters?
    • Barrier layer cells
    • Photocell
  20. Contain a negatively charged cathode and a positively charged anode enclosed in a glass case. The cathode is composed of a material that acts as a resistor in the dark but emitts electrons when exposed to light.
Card Set
Clinical Chemistry