BIO CELL 371 E1 C5

WHAT IS THE VCISABLE RANGE ?

380- 760 nm
EXAMPLE OF SHORT WAVELENGTHS

UV
EXAMPLE OF LONG WAVELENGTHS

INFARED (IR)
QUALITATIVE VS QUANTITATIVE
- QUALITATIVE: PRESENT OR NOT
- QUANTITATIVE: HOW MUCH
DEFINE ABSORPTION MAXIMA

THE WAVELENGTHS AT WHICH THE ABSORPTION SPECTRIUM EXHIBITS A PEAK.
WHAT'S THE SPEC 20 RANGE?
- 340-1000nm
- NEAR UV - VISIABLE-NEAR IR
HOW DOES THE SPEC 20 WORK?
- TUNGSTON LAMP-->
- DEFRACTION GRATING (ADJUST VIA CAM)-->
- 20nm BEAM HITS SAMPLE---->
- PHOTOTUBE READS BEAM.
- ABS AND TRANS READOUT.
DEFINE TRANSMISSION
- THE FRACTION OF INCIDENT OR INCOMING LIGHT TRANSMITTED BY A SAMPLE.
- T = I/Io X 100
- I=STRIKE/Io=AMOUNT AFTER STRIKE.( IN %)
IF YOU HAD T HOW DO YOU GET A?
- A = LOG 1/T
- OR LOG Io/I
- (NOTE THAT THE LOG FORMULA IS INVERSE OF T FORMULA)
AT 100% TRANSMITTANCE, WHAT WOULD A BE?
- 0
- A= LOG 1=0
HIGH T = LOW A
WHAT ARE THE SCALES FOR T% AND A?
- T% = 100 TO 0
- A = 0 TO INFINITY
WHY PLOT A AND NOT T%

USUALLY PLOT A AGAINST CONCENTRATION, IT GIVE A LINEAR LINE. T% GIVES A CURVE.
WHEN YOU START, YOU HAVE A QUARTZ CUVETTE AND YOUR SOLVANT INSIDE OF IT, EXECPT FOR WHAT YOUR MEASURING. WHAT IS THE SETTING OF T% AND A ?
- T% = 100
- AND THUS
- A = 0
DEFINE BEER-LAMBERT LAW.

ABSORPTION IS DIRECTLY PROPORTIONAL TO SOLUTE CONCENTRATION (C) AND THE LENGTH OF THE LIGHT PATH (L).
WHAT IS THE BEER-LAMBERT FORMULA?
- E = ABSORPTION COEFFICENT
- E & C (CONCENTRATION) = GIVEN IN UNITS OF WEIGHT.
- L = 1cm (USUALLY)
EGG WHITES CONTAIN BOTH _______ & _______.
BOTH ARE SOLUBILE IN ________.
BUT ONLY _________ ARE SOLUBLE IN _______.
- ALBUMINS & GLOBULINS
- SALINE (.85% NaCl)
- ALBUMINS ARE SOLUBILE IN WATER.

Author

shockwave

138319

Card Set

BIO CELL 371 E1 C5

Description

BIO CELL 371 E1 C5 GSU 2012

Updated

2012-03-20T15:50:24Z

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