#13 Microarrays

Gene Expression Profiling is used to assess ____________ within a sample simultaneously

Gene Expression Profiling uses a targeted set of __________

__________ is a test of breast cancer tumor tissue to predict ___________ and ___________

CYP450 analysis uses a targeted set of ______________

Genes selected for the Oncotype DX Breast Cancer Assay include ____________, ___________, & ___________

expression of a gene correlates with ___________ and/or ____________

PCR products are genenrally ____________ labelled and
____________ to the 21 gene chip.

Stage I & II cancer is _______ which means it is ________

Algorithms are created for ________ and ________

Tissue samples for the Oncotype DX Breast Cancer Assay do not need to be fresh. They can be taken from _____________

PCR products are genenrally fluorescently labelled and hybridized to the _____________.

The chip is read by laser for _____________

One can analyze for a restricted set of genes at the _____ level using ________ __________ __________ __________.

Amplichip CYP450 is a commercial chip to interrogate samples for _______ and ________ polymorphisms

Amplichip CYP450 can use either _______ or _______ DNA samples.

Which DNA sample type is reccommended for Amplichip CYP450

Amplichip CYP450 looks at ______CYP2D6 polymorphisms and ______ CYP2C19 polymorphisms

________ for the Amplichip CYP450 flow is extracted from the patient sample

DNA for the Amplichip CYP450 flow is amplified by ________

Products for the Amplichip CYP450 are _________ and __________ labelled

The sample _______ is __________ to the gene chip.

microRNA do not have ________

Protein Expression Profiling uses a series of ________ fixed to a ______________ to detect the presence of ________ within a sample.

____________ is useful for examining a number of signal transduction pathways simultaneously while exposing cells or tissues to drug

________ uses whole tissue instead of isolating proteins to detect where the ligand is bound and how much

________ looks at 3 markers nad how the makers look different on different samples and at different _______

Protein Expression Profiling is useful for examining a number of ___________ simultaneously while exposing cells or tissues to ___________

CHiP stands for

ChiP is an assay that is used to identify the ________ __________ and ________ _________
associated with specific genes.

ChIP-on-chip can label ________ products with ________ and _______ from two cell conditions and expose to a gene microarray

What two parameters define microarray detectors

A resolution of _______ is needed in a detector system to detect 1:500,000 copies

The detector system needs a sensitivity of _______

What are the two types of detector systems

What do the two types of detector systems have in common

___________ collect laser light on _________ and then reconstruct the entire field

_________ use a __________ to collect a picture of the light pattern

Detection probes generally use a __________ tag, particularly the cyanine dyes ______ and ______

Some problems with detection probes are ______ and _______

Dual fluorescence/chemiluminescence –
here each spot contains two ______ , one labeled with _______ for chemiluminescnce, the second labeled with _____________.

Dual fluorescence/chemiluminescence is used to compare _______ ________ to ensure reading spot correctly. This should decrease ________ ________