GEN #10 DNA Sequencing and Mutagenesis

the most widely used DNA sequencing method

Two general methods used for sequencing

Which sequencing method uses chemical degradation of the original DNA

The _________ sequencing method is used to confirm the _______ sequencing method

Which sequencing method is used for analysis of DNA modifications

The Sanger Method uses a ____________ to terminate chain elongation at a specific nucleotide

This mutagenesis method uses a ________ that has the appropriate mismatch located _________

Oligonucleotide-mediated mutagenesis requires what 3 reagents

Allows one to insert a small sequence of altered bases into a sequence

compression of the sequence occurs as the replicated DNA gets _________

Which sequencing method readily gives nucleotide base sequences but not modifications

sequencing details read in what direction

This mutagenesis method is limited to the mutation of just a few nucleotides at a time

This mutagenesis method uses a universal primer

PCR-mediated mutagenesis uses a ____________ that has the appropriate mismatch located within it

most DNA sequencing uses the ________ method

Sanger cannot sequence areas that induce _______

Oligonucleotide-mediated mutagenesis prefers _________ DNA over __________ DNA.

___________ changes DNA sequences at a chosen location

The flanking region has a sequence that is __________ to the ______________