1. What factors affect microbial growth?
    food, water, temperature, pH, oxygen and carbon dioxide
  2. What do we use microbial growth for?
    to identify pathogens in the lab
  3. What are three types of microorganisms that have a specific temperature need?
    • Thermophiles (~50C and up) hotter than body
    • Mesophiles (~25C to ~40C) in body
    • Psychrophiles (~ -5C to ~20C) in fridge
  4. Which type of temperature specific microorganism is normal flora and why?
    • Mesophiles
    • prefer our body temp and pH
  5. What is used to preserve some foods and how?
    Salt eliminates bacteria's water source
  6. What is a type of microorganism that loves salt?
  7. What do obligate or extreme halophiles need?
  8. Where in humans would you find halophiles?
    on skin or in the eyes
  9. Which microorganism is salt tolerant and sometimes a human pathogen?
    facultative halophile
  10. What are some oxygen associated microorganisms?
    Facultative Anaerobes / Facultative Aerobes
  11. Which organism can not survive without oxygen and why?
    • obligate aerobes
    • need oxygen for aerobic respiration
  12. Which organism needs small amounts of oxygen?
  13. Which microorganism needs an environment without oxygen and how do they make energy?
    • obligate anerobe
    • fermentation
  14. Which organism is oxygen tolerant because they can't use oxygen for energy production?
  15. Which organism is flexible with or without oxygen and can use oxygen for aerobic respiration?
    Facultative Anaerobes / Facultative Aerobes
  16. What organisms needs carbon dioxide?
  17. What organism causes food poisoning and include periodontal pathogens?
  18. What is campylobacteria?
    food poisoning
  19. What types of pathogens are capnophiles known for?
    neissa (gonorrhea and Miningitis)
  20. What phase is the time it takes for one organism to double or reproduce?
    generation / doubling time
  21. What is the average bacteria's generation time?
    30min - 1hr
  22. Why are slow growers negative?
    • take up to 1 day for bacteria to reproduce
    • symptoms don't show, disease can be shared
    • lab can't diagnose soon
  23. How do you find the generation time?
    Make a table with number of organisms from the start on one side and time it took for organisms to reproduce/double once on the other side
  24. What are the phases of microorganism growth?
    • 1. Lag Phase
    • 2. Log Phase
    • 3. Stationary Phase
    • 4. Death Phase (also known as Decline)
  25. What phase shows the time bacteria takes before doubling?
    lag phase
  26. What phase shows the exponential growth?
    log phase
  27. What phase show a steady reproduction and death that balances out so there is no higher number of microorganisms?
    stationary phase
  28. What phase shows organisms dying?
    death phase/ decline
Card Set
micro growth