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What factors affect microbial growth?

food, water, temperature, pH, oxygen and carbon dioxide
What do we use microbial growth for?

to identify pathogens in the lab
What are three types of microorganisms that have a specific temperature need?
- Thermophiles (~50C and up) hotter than body
- Mesophiles (~25C to ~40C) in body
- Psychrophiles (~ -5C to ~20C) in fridge
Which type of temperature specific microorganism is normal flora and why?
- Mesophiles
- prefer our body temp and pH
What is used to preserve some foods and how?

Salt eliminates bacteria's water source
What is a type of microorganism that loves salt?

Halophiles
What do obligate or extreme halophiles need?

salt
Where in humans would you find halophiles?

on skin or in the eyes
Which microorganism is salt tolerant and sometimes a human pathogen?

facultative halophile
What are some oxygen associated microorganisms?

Facultative Anaerobes / Facultative Aerobes
Which organism can not survive without oxygen and why?
- obligate aerobes
- need oxygen for aerobic respiration
Which organism needs small amounts of oxygen?

microaerophiles
Which microorganism needs an environment without oxygen and how do they make energy?
- obligate anerobe
- fermentation
Which organism is oxygen tolerant because they can't use oxygen for energy production?

aerotolerant
Which organism is flexible with or without oxygen and can use oxygen for aerobic respiration?

Facultative Anaerobes / Facultative Aerobes
What organisms needs carbon dioxide?

capnophiles
What organism causes food poisoning and include periodontal pathogens?

capnophiles
What is campylobacteria?

food poisoning
What types of pathogens are capnophiles known for?

neissa (gonorrhea and Miningitis)
What phase is the time it takes for one organism to double or reproduce?

generation / doubling time
What is the average bacteria's generation time?

30min - 1hr
Why are slow growers negative?
- take up to 1 day for bacteria to reproduce
- symptoms don't show, disease can be shared
- lab can't diagnose soon
How do you find the generation time?

Make a table with number of organisms from the start on one side and time it took for organisms to reproduce/double once on the other side
What are the phases of microorganism growth?
- 1. Lag Phase
- 2. Log Phase
- 3. Stationary Phase
- 4. Death Phase (also known as Decline)
What phase shows the time bacteria takes before doubling?

lag phase
What phase shows the exponential growth?

log phase
What phase show a steady reproduction and death that balances out so there is no higher number of microorganisms?

stationary phase
What phase shows organisms dying?

death phase/ decline

Author

eingram

135812

Card Set

4microQA

Description

micro growth

Updated

2012-05-02T00:00:43Z

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