Media types

• basic
• enriched
• differential
• selective

has basic nutrients to allow growth

most bacteria of clinical interest will grow

has additional stuff for organisms that need more

tell organism different because of what happened to plate hemolysis, color change

encourages or inhibits growth of specific organisms

• Enriched and Differential
• contains 5% sheep blood
• alpha, beta, and gamma Hemolysis

RBC destroyed

• selective and differential
• selects for halophiles--salt lovers grow in salt environment
• differentiates between
• S. Aureua ---- changes from pink to yellow
• S. epidermidis---- does not change color
• inhibits growth of nonhalophiles and encourages growth of halophiles
• phenol red ph indicator

causes an area of green to appear in the agar surrounding the bacterial colonies

alpha hemolysis is incomplete hemolysis

causes a clear area to appear in the agar surrounding the bacterial colonies

is complete lysing of blood content by colony

occurs when there is no hemolysis present int he agar surrounding the bacterial colonies

no hemolysis

• selective, differential
• bile salts, crystal violet, lactose, neutral red indicator
• inhibits gram positives
• lactose fermenters turn red/pink
• neutral red indicator
• biles salts, and crystal violet inhibit growth of gram positive bacteria

• phenylethanol
• 5% sheep blood
• smells like flowers
• inhibits anaerobic gram neg bacilli
• supports gram positives

• changes from green to blue in alkaline environment
• bile salts, lactose, sucrose alicin, ferric ammonium citrate, bromthymol blue and acid fuchsin indicators (ph indicators)
• green media
• selective differential
• salmonella black center
• shigella no black center
• supports gram neg

• light brown in slant
• ecoli will grow with no color change
• bile salts, esculin,ferric citrate
• selective, differential
• bile turn the medium black
• enterococcus opportnisists only to grow this

• green
• citrate bromthymol blue indicator
• salmonella pseudomonas
• change from green to bright blue
• selective and differential